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Title: MicroED data collection and processing

The collection and processing of MicroED data are presented. MicroED, a method at the intersection of X-ray crystallography and electron cryo-microscopy, has rapidly progressed by exploiting advances in both fields and has already been successfully employed to determine the atomic structures of several proteins from sub-micron-sized, three-dimensional crystals. A major limiting factor in X-ray crystallography is the requirement for large and well ordered crystals. By permitting electron diffraction patterns to be collected from much smaller crystals, or even single well ordered domains of large crystals composed of several small mosaic blocks, MicroED has the potential to overcome the limiting size requirement and enable structural studies on difficult-to-crystallize samples. This communication details the steps for sample preparation, data collection and reduction necessary to obtain refined, high-resolution, three-dimensional models by MicroED, and presents some of its unique challenges.
Authors:
; ; ; ;  [1] ;  [2] ;  [1]
  1. Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147 (United States)
  2. Medical Research Council Laboratory of Molecular Biology, Cambridge (United Kingdom)
Publication Date:
OSTI Identifier:
22389064
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section A, Foundations and Advances (Online); Journal Volume: 71; Journal Issue: Pt 4; Other Information: PMCID: PMC4487423; PMID: 26131894; PUBLISHER-ID: mq5031; OAI: oai:pubmedcentral.nih.gov:4487423; Copyright (c) Johan Hattne et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLOGRAPHY; CRYSTALS; ELECTRON DIFFRACTION; ELECTRONS; MICROSCOPY; NANOSTRUCTURES; POTENTIALS; PROCESSING; PROTEINS; REDUCTION; RESOLUTION; SAMPLE PREPARATION