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Title: Practical macromolecular cryocrystallography

Current methods, reagents and experimental hardware for successfully and reproducibly flash-cooling macromolecular crystals to cryogenic temperatures for X-ray diffraction data collection are reviewed. Cryocrystallography is an indispensable technique that is routinely used for single-crystal X-ray diffraction data collection at temperatures near 100 K, where radiation damage is mitigated. Modern procedures and tools to cryoprotect and rapidly cool macromolecular crystals with a significant solvent fraction to below the glass-transition phase of water are reviewed. Reagents and methods to help prevent the stresses that damage crystals when flash-cooling are described. A method of using isopentane to assess whether cryogenic temperatures have been preserved when dismounting screened crystals is also presented.
Authors:
 [1]
  1. Rigaku Americas Corp., 9009 New Trails Drive, The Woodlands, TX 77381 (United States)
Publication Date:
OSTI Identifier:
22375721
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta crystallographica. Section F, Structural biology communications; Journal Volume: 71; Journal Issue: Pt 6; Other Information: PMCID: PMC4461322; PMID: 26057787; PUBLISHER-ID: en5564; PUBLISHER-ID: S2053230X15008304; OAI: oai:pubmedcentral.nih.gov:4461322; Copyright (c) Pflugrath 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ANNEALING; COOLING; CURRENTS; DAMAGE; GLASS; MONOCRYSTALS; PRESSURE RANGE MEGA PA 10-100; SOLVENTS; STRESSES; WATER; X-RAY DIFFRACTION