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Title: The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster

The highly conserved domain of unknown function in the cyanobactin superfamily has a novel fold. The protein does not appear to bind the most plausible substrates, leaving questions as to its role. Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated.
Authors:
; ; ;  [1] ;  [2] ;  [3] ;  [4] ;  [2] ; ;  [1]
  1. University of St Andrews, North Haugh, St Andrews, Fife KY16 8ST, Scotland (United Kingdom)
  2. University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, Scotland (United Kingdom)
  3. (United Kingdom)
  4. (Egypt)
Publication Date:
OSTI Identifier:
22375708
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta crystallographica. Section F, Structural biology communications; Journal Volume: 70; Journal Issue: Pt 12; Other Information: PMCID: PMC4259220; PMID: 25484206; PUBLISHER-ID: sx5117; PUBLISHER-ID: S2053230X1402425X; OAI: oai:pubmedcentral.nih.gov:4259220; Copyright (c) Mann et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTAL STRUCTURE; INTERACTIONS; SUBSTRATES