skip to main content

Title: Applications of the second virial coefficient: protein crystallization and solubility

This article highlights some of the ground-based studies emanating from NASA’s Microgravity Protein Crystal Growth (PCG) program, and includes a more detailed discussion of the history and the progress made in one of the NASA-funded PCG investigations involving the use of measured second virial coefficients (B values) as a diagnostic indicator of solution conditions conducive to protein crystallization. This article begins by highlighting some of the ground-based studies emanating from NASA’s Microgravity Protein Crystal Growth (PCG) program. This is followed by a more detailed discussion of the history of and the progress made in one of the NASA-funded PCG investigations involving the use of measured second virial coefficients (B values) as a diagnostic indicator of solution conditions conducive to protein crystallization. A second application of measured B values involves the determination of solution conditions that improve or maximize the solubility of aqueous and membrane proteins. These two important applications have led to several technological improvements that simplify the experimental expertise required, enable the measurement of membrane proteins and improve the diagnostic capability and measurement throughput.
Authors:
 [1] ;  [2] ;  [3]
  1. Mississippi State University, Starkville, MS 39759 (United States)
  2. University of Alabama at Birmingham, 1720 Second Avenue South, Birmingham, AL 35294 (United States)
  3. (United States)
Publication Date:
OSTI Identifier:
22375685
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta crystallographica. Section F, Structural biology communications; Journal Volume: 70; Journal Issue: Pt 5; Other Information: PMCID: PMC4014317; PMID: 24817708; PUBLISHER-ID: en5549; OAI: oai:pubmedcentral.nih.gov:4014317; Copyright (c) Wilson & DeLucas 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTAL GROWTH; CRYSTALLIZATION; MATHEMATICAL SOLUTIONS; SOLUBILITY; SOLUTIONS