Crystallization and preliminary X-ray characterization of the genetically encoded fluorescent calcium indicator protein GCaMP2
- Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus (Puerto Rico)
- Department of Chemistry, University of Puerto Rico, Rio Piedras Campus (Puerto Rico)
- Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia (United States)
The genetically encoded fluorescent calcium-indicator protein GCaMP2 was crystallized in the calcium-saturated form. X-ray diffraction data were collected to 2.0 Å resolution and the structure was solved by molecular replacement. Fluorescent proteins and their engineered variants have played an important role in the study of biology. The genetically encoded calcium-indicator protein GCaMP2 comprises a circularly permuted fluorescent protein coupled to the calcium-binding protein calmodulin and a calmodulin target peptide, M13, derived from the intracellular calmodulin target myosin light-chain kinase and has been used to image calcium transients in vivo. To aid rational efforts to engineer improved variants of GCaMP2, this protein was crystallized in the calcium-saturated form. X-ray diffraction data were collected to 2.0 Å resolution. The crystals belong to space group C2, with unit-cell parameters a = 126.1, b = 47.1, c = 68.8 Å, β = 100.5° and one GCaMP2 molecule in the asymmetric unit. The structure was phased by molecular replacement and refinement is currently under way.
- OSTI ID:
- 22360584
- Journal Information:
- Acta Crystallographica. Section F, Vol. 64, Issue Pt 7; Other Information: PMCID: PMC2443961; PMID: 18607093; PUBLISHER-ID: bw5243; OAI: oai:pubmedcentral.nih.gov:2443961; Copyright (c) International Union of Crystallography 2008; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
Similar Records
Purification, crystallization and preliminary X-ray diffraction of human S100A15
Genetically encoded sensors enable real-time observation of metabolite production