Crystallization and preliminary X-ray diffraction analysis of maize aldose reductase

Journal Article · Thu Nov 01 00:00:00 EDT 2007 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309107052670· OSTI ID:22360424

Kiyota, Eduardo ^[1]; Sousa, Sylvia Morais de ^[2]; Santos, Marcelo Leite dos; Costa Lima, Aline da ^[1]; Menossi, Marcelo ^[3]; Yunes, José Andrés ^[4]; Aparicio, Ricardo ^[1]

---

1. Laboratório de Biologia Estrutural, Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13083-970 Campinas-SP (Brazil)
2. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas-SP (Brazil)
3. Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas-SP (Brazil)
4. Laboratório de Biologia Molecular, Centro Infantil Boldrini, Campinas-SP (Brazil)

Preliminary X-ray diffraction studies of apo maize aldose reductase at 2.0 Å resolution are reported. Maize aldose reductase (AR) is a member of the aldo-keto reductase superfamily. In contrast to human AR, maize AR seems to prefer the conversion of sorbitol into glucose. The apoenzyme was crystallized in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 47.2, b = 54.5, c = 100.6 Å and one molecule in the asymmetric unit. Synchrotron X-ray diffraction data were collected and a final resolution limit of 2.0 Å was obtained after data reduction. Phasing was carried out by an automated molecular-replacement procedure and structural refinement is currently in progress. The refined structure is expected to shed light on the functional/enzymatic mechanism and the unusual activities of maize AR.

• OSTI ID: 22360424
• Journal Information: Acta Crystallographica. Section F, Vol. 63, Issue Pt 11; Other Information: PMCID: PMC2339750; PMID: 18007059; PUBLISHER-ID: fw5152; OAI: oai:pubmedcentral.nih.gov:2339750; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CONVERSION
CRYSTALLIZATION
GLUCOSE
MOLECULES
REDUCTION
RESOLUTION
SPACE GROUPS
SYNCHROTRONS
VISIBLE RADIATION
X-RAY DIFFRACTION