Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase

Journal Article · Thu Nov 01 00:00:00 EDT 2007 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309107047069· OSTI ID:22360417

Morita, Hiroyuki ^[1]; Kondo, Shin; Kato, Ryohei ^[2]; Wanibuchi, Kiyofumi; Noguchi, Hiroshi ^[3]; Sugio, Shigetoshi ^[2]; Abe, Ikuro ^[3]; Kohno, Toshiyuki ^[1]

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1. Mitsubishi Kagaku Institute of Life Sciences (MITILS), 11 Minamiooya, Machida, Tokyo 194-8511 (Japan)
2. Innovation Center Yokohama, Mitsubishi Chemical Corporation, 1000 Kamoshida, Aoba, Yokohama, Kanagawa 227-8502 (Japan)
3. School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526 (Japan)

Octaketide synthase from A. arborescens has been overexpressed in E. coli, purified and crystallized. Diffraction data have been collected to 2.6 Å. Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group I422, with unit-cell parameters a = b = 110.2, c = 281.4 Å, α = β = γ = 90.0°. Diffraction data were collected to 2.6 Å resolution using synchrotron radiation at BL24XU of SPring-8.

• OSTI ID: 22360417
• Journal Information: Acta Crystallographica. Section F, Vol. 63, Issue Pt 11; Other Information: PMCID: PMC2339743; PMID: 18007047; PUBLISHER-ID: bo5031; OAI: oai:pubmedcentral.nih.gov:2339743; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
DIFFRACTION
DIFFUSION
ESCHERICHIA COLI
MOLECULES
RESOLUTION
SPACE GROUPS
SYNCHROTRON RADIATION