Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi
Abstract
Old yellow enzyme from Trypanosoma cruzi, has been crystallized using the hanging-drop vapour-diffusion method. Old yellow enzyme (OYE) is an NADPH oxidoreductase that contains a flavin mononucleotide as a prosthetic group. The OYE from Trypanosoma cruzi, which produces prostaglandin F{sub 2α}, a potent mediator of various physiological and pathological processes, from prostaglandin H2. The protein was recombinantly expressed and purified from Escherichia coli and was crystallized using the hanging-drop vapour-diffusion method. The crystal belongs to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 56.3, b = 78.8, c = 78.8 Å, β = 93.4° and two molecules per asymmetric unit. The crystals were suitable for X-ray crystallographic studies and diffracted to 1.70 Å resolution. A Patterson search method is in progress using the structure of OYE from Pseudomonas putida as a starting model.
- Authors:
-
- Maruwa Foods Co. Ltd, Tsutsui-cho 170-1, Yamatokoriyama, Nara 639-1123 (Japan)
- Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamada-Oka, Suita, Osaka 565-0871 (Japan)
- Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Osaka 565-0874 (Japan)
- Publication Date:
- OSTI Identifier:
- 22360410
- Resource Type:
- Journal Article
- Journal Name:
- Acta Crystallographica. Section F
- Additional Journal Information:
- Journal Volume: 63; Journal Issue: Pt 10; Other Information: PMCID: PMC2339734; PMID: 17909300; PUBLISHER-ID: pu5199; OAI: oai:pubmedcentral.nih.gov:2339734; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALS; DIFFUSION; ESCHERICHIA COLI; MOLECULES; RESOLUTION; SPACE GROUPS
Citation Formats
Sugiyama, Shigeru, Tokuoka, Keiji, Uchiyama, Nahoko, Okamoto, Naoki, Okano, Yousuke, Matsumura, Hiroyoshi, Inaka, Koji, Urade, Yoshihiro, Inoue, Tsuyoshi, and Maruwa Foods Co. Ltd, Tsutsui-cho 170-1, Yamatokoriyama, Nara 639-1123. Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi. United Kingdom: N. p., 2007.
Web. doi:10.1107/S1744309107044879.
Sugiyama, Shigeru, Tokuoka, Keiji, Uchiyama, Nahoko, Okamoto, Naoki, Okano, Yousuke, Matsumura, Hiroyoshi, Inaka, Koji, Urade, Yoshihiro, Inoue, Tsuyoshi, & Maruwa Foods Co. Ltd, Tsutsui-cho 170-1, Yamatokoriyama, Nara 639-1123. Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi. United Kingdom. https://doi.org/10.1107/S1744309107044879
Sugiyama, Shigeru, Tokuoka, Keiji, Uchiyama, Nahoko, Okamoto, Naoki, Okano, Yousuke, Matsumura, Hiroyoshi, Inaka, Koji, Urade, Yoshihiro, Inoue, Tsuyoshi, and Maruwa Foods Co. Ltd, Tsutsui-cho 170-1, Yamatokoriyama, Nara 639-1123. 2007.
"Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi". United Kingdom. https://doi.org/10.1107/S1744309107044879.
@article{osti_22360410,
title = {Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi},
author = {Sugiyama, Shigeru and Tokuoka, Keiji and Uchiyama, Nahoko and Okamoto, Naoki and Okano, Yousuke and Matsumura, Hiroyoshi and Inaka, Koji and Urade, Yoshihiro and Inoue, Tsuyoshi and Maruwa Foods Co. Ltd, Tsutsui-cho 170-1, Yamatokoriyama, Nara 639-1123},
abstractNote = {Old yellow enzyme from Trypanosoma cruzi, has been crystallized using the hanging-drop vapour-diffusion method. Old yellow enzyme (OYE) is an NADPH oxidoreductase that contains a flavin mononucleotide as a prosthetic group. The OYE from Trypanosoma cruzi, which produces prostaglandin F{sub 2α}, a potent mediator of various physiological and pathological processes, from prostaglandin H2. The protein was recombinantly expressed and purified from Escherichia coli and was crystallized using the hanging-drop vapour-diffusion method. The crystal belongs to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 56.3, b = 78.8, c = 78.8 Å, β = 93.4° and two molecules per asymmetric unit. The crystals were suitable for X-ray crystallographic studies and diffracted to 1.70 Å resolution. A Patterson search method is in progress using the structure of OYE from Pseudomonas putida as a starting model.},
doi = {10.1107/S1744309107044879},
url = {https://www.osti.gov/biblio/22360410},
journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 10,
volume = 63,
place = {United Kingdom},
year = {Mon Oct 01 00:00:00 EDT 2007},
month = {Mon Oct 01 00:00:00 EDT 2007}
}