Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2
- Life Science Group, Research Division, National Synchrotron Radiation Research Center, Hsinchu 30076,Taiwan (China)
- Department of Applied Chemistry, National Chiao Tung University, Hsinchu 30010,Taiwan (China)
The crystallization of B. cereus chitinase is reported. Chitinases (EC 3.2.1.14) are found in a broad range of organisms, including bacteria, fungi and higher plants, and play different roles depending on their origin. A chitinase from Bacillus cereus NCTU2 (ChiNCTU2) capable of hydrolyzing chitin as a carbon and nitrogen nutrient has been identified as a member of the family 18 glycoside hydrolases. ChiNCTU2 of molecular weight 36 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of chitinase crystals at 1.10 Å resolution, the crystal belongs to space group P2{sub 1}, with unit-cell parameters a = 50.79, b = 48.79, c = 66.87 Å, β = 99.31°. Preliminary analysis indicates there is one chitinase molecule in the asymmetric unit, with a solvent content of 43.4%.
- OSTI ID:
- 22360162
- Journal Information:
- Acta Crystallographica. Section F, Vol. 62, Issue Pt 9; Other Information: PMCID: PMC2242883; PMID: 16946479; PUBLISHER-ID: pu5155; OAI: oai:pubmedcentral.nih.gov:2242883; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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