Crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis CRP/FNR family transcription regulator
- Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500076 (India)
The CRP/FNR family transcription factor from M. tuberculosis H37Rv has been crystallized in space group P2{sub 1}2{sub 1}2{sub 1} in the absence of cAMP. The crystals show the presence of a dimeric molecule in the asymmetric unit. CRP/FNR family members are transcription factors that regulate the transcription of many genes in Escherichia coli and other organisms. Mycobacterium tuberculosis H37Rv contains a probable CRP/FNR homologue encoded by the open reading frame Rv3676. The deletion of this gene is known to cause growth defects in cell culture, in bone marrow-derived macrophages and in a mouse model of tuberculosis. The mycobacterial gene Rv3676 shares ∼32% sequence identity with prototype E. coli CRP. The structure of the protein might provide insight into transcriptional regulation in the pathogen by this protein. The M. tuberculosis CRP/FNR transcription regulator was crystallized in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 54.1, b = 84.6, c = 101.2 Å. The crystal diffracted to a resolution of 2.9 Å. Matthews coefficient and self-rotation function calculations reveal the presence of two monomers in the asymmetric unit.
- OSTI ID:
- 22360159
- Journal Information:
- Acta Crystallographica. Section F, Vol. 62, Issue Pt 9; Other Information: PMCID: PMC2242880; PMID: 16946467; PUBLISHER-ID: ll5077; OAI: oai:pubmedcentral.nih.gov:2242880; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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