Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form
- Departamento Química-Física, Bioquímica y Química Inorgánica, Universidad de Almería, Carretera Sacramento, Almería 04120 (Spain)
- Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061 (United States)
The crystallization of GADPH from S. oleracea in two different space groups has been accomplished using GAPDH samples with unusually low purity levels. One crystal form is the same as a previously reported form, while the second represents a new form. The quality of the diffraction allowed the structure to be determined at a 3 Å resolution limit. Two crystalline forms of GADPH (d-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 Å and the other of which belonged to a new space group I4{sub 1}22, with unit-cell parameters a = b = 120.9, c = 154.5 Å. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 Å. Differences at the NAD{sup +}/NADP{sup +}-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme.
- OSTI ID:
- 22356403
- Journal Information:
- Acta Crystallographica. Section F, Vol. 62, Issue Pt 11; Other Information: PMCID: PMC2225226; PMID: 17077485; PUBLISHER-ID: pu5159; OAI: oai:pubmedcentral.nih.gov:2225226; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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