Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase
- School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555 (Japan)
- Gifu Pharmaceutical University, Gifu 502-8585 (Japan)
- Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973 (Japan)
Pig heart carbonyl reductase has been crystallized in the presence of NADPH. Diffraction data have been collected using synchrotron radiation. Pig heart carbonyl reductase (PHCR), which belongs to the short-chain dehydrogenase/reductase (SDR) family, has been crystallized by the hanging-drop vapour-diffusion method. Two crystal forms (I and II) have been obtained in the presence of NADPH. Form I crystals belong to the tetragonal space group P4{sub 2}, with unit-cell parameters a = b = 109.61, c = 94.31 Å, and diffract to 1.5 Å resolution. Form II crystals belong to the tetragonal space group P4{sub 1}2{sub 1}2, with unit-cell parameters a = b = 120.10, c = 147.00 Å, and diffract to 2.2 Å resolution. Both crystal forms are suitable for X-ray structure analysis at high resolution.
- OSTI ID:
- 22356358
- Journal Information:
- Acta Crystallographica. Section F, Vol. 62, Issue Pt 10; Other Information: PMCID: PMC2225176; PMID: 17012807; PUBLISHER-ID: en5195; OAI: oai:pubmedcentral.nih.gov:2225176; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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