Crystallization and halide phasing of the C-terminal domain of human KIN17
- Laboratoire de Cristallographie, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne (Switzerland)
- Laboratoire de Marquage des Protéines, Département d’Ingénierie et d’Etude des Protéines, Commissariat a l’Energie Atomique, 91191 Gif-sur-Yvette (France)
- Laboratoire de Structure des Protéines, Département d’Ingénierie et d’Etude des Protéines, Commissariat a l’Energie Atomique, 91191 Gif-sur-Yvette (France)
Expression, purification, crystallization and phasing procedure are reported for the C-terminal domain of human KIN17. Here, the crystallization and initial phasing of the C-terminal domain of human KIN17, a 45 kDa protein mainly expressed in response to ionizing radiation and overexpressed in certain tumour cell lines, are reported. Crystals diffracting to 1.4 Å resolution were obtained from 10% ethylene glycol, 27% PEG 6000, 500 mM LiCl and 100 mM sodium acetate pH 6.3 in space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 45.75, b = 46.31, c = 60.80 Å and one molecule in the asymmetric unit. Since this domain has a basic pI, heavy-atom derivatives were obtained by soaking the crystals with negatively charged ions such as tungstate and iodine. The replacement of LiCl by KI in the cryosolution allowed the determination of phases from iodide ions to give an interpretable electron-density map.
- OSTI ID:
- 22356299
- Journal Information:
- Acta Crystallographica. Section F, Vol. 62, Issue Pt 3; Other Information: PMCID: PMC2197188; PMID: 16511313; PUBLISHER-ID: ll5048; OAI: oai:pubmedcentral.nih.gov:2197188; Copyright (c) International Union of Crystallography 2006; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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