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Title: X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater

Abstract

The protein C-phycocyanin, involved in photosynthesis, has been purified from three cyanobacterial species: Spirulina, Phormidium and Lyngbya. These three proteins have been crystallized and characterized using X-ray crystallography. C-phycocyanins from three cyanobacterial cultures of freshwater and marine habitat, Spirulina, Phormidium and Lyngbya spp., were purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Blue-coloured crystals in different crystal forms, monoclinic and hexagonal, were obtained for the three species. The crystals took 1–12 weeks to grow to full size using polyethylene glycols of different molecular weights as precipitants. The amino-acid sequences of these proteins show high similarity to other known C-phycocyanins from related organisms; however, the C-phycocyanins reported here showed different biochemical and biophysical properties, i.e. molecular weight, stability etc. The X-ray diffraction data were collected at resolutions of 3.0 Å for the monoclinic and 3.2 and 3.6 Å for the hexagonal forms. The unit-cell parameters corresponding to the monoclinic space group P2{sub 1} are a = 107.33, b = 115.64, c = 183.26 Å, β = 90.03° for Spirulina sp. C-phycocyanin and are similar for crystals of Phormidium and Lyngbya spp. C-phycocyanins. Crystals belonging to the hexagonal space group P6{sub 3}, with unit-cell parameters a = b = 154.97,more » c = 40.35 Å and a = b = 151.96, c = 39.06 Å, were also obtained for the C-phycocyanins from Spirulina and Lyngbya spp., respectively. The estimated solvent content is around 50% for the monoclinic crystals of all three species assuming the presence of two hexamers per asymmetric unit. The solvent content is 66.5 and 64.1% for the hexagonal crystals of C-phycocyanin from Spirulina and Lyngbya spp. assuming the presence of one αβ monomer per asymmetric unit.« less

Authors:
; ; ;  [1]
  1. Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, Gujarat (India)
Publication Date:
OSTI Identifier:
22356156
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 61; Journal Issue: Pt 9; Other Information: PMCID: PMC1978106; PMID: 16511175; PUBLISHER-ID: za5111; OAI: oai:pubmedcentral.nih.gov:1978106; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLOGRAPHY; CRYSTALS; DIFFUSION; MOLECULAR WEIGHT; MONOMERS; PHOTOSYNTHESIS; RESOLUTION; SOLVENTS; SPACE GROUPS; STABILITY; X-RAY DIFFRACTION

Citation Formats

Satyanarayana, L., Suresh, C. G., E-mail: cgsuresh@ncl.res.in, Patel, Anamika, Mishra, Sandhya, Ghosh, Pushpito Kumar, and Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, Maharashtra. X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater. United Kingdom: N. p., 2005. Web. doi:10.1107/S1744309105025649.
Satyanarayana, L., Suresh, C. G., E-mail: cgsuresh@ncl.res.in, Patel, Anamika, Mishra, Sandhya, Ghosh, Pushpito Kumar, & Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, Maharashtra. X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater. United Kingdom. https://doi.org/10.1107/S1744309105025649
Satyanarayana, L., Suresh, C. G., E-mail: cgsuresh@ncl.res.in, Patel, Anamika, Mishra, Sandhya, Ghosh, Pushpito Kumar, and Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, Maharashtra. 2005. "X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater". United Kingdom. https://doi.org/10.1107/S1744309105025649.
@article{osti_22356156,
title = {X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater},
author = {Satyanarayana, L. and Suresh, C. G., E-mail: cgsuresh@ncl.res.in and Patel, Anamika and Mishra, Sandhya and Ghosh, Pushpito Kumar and Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, Maharashtra},
abstractNote = {The protein C-phycocyanin, involved in photosynthesis, has been purified from three cyanobacterial species: Spirulina, Phormidium and Lyngbya. These three proteins have been crystallized and characterized using X-ray crystallography. C-phycocyanins from three cyanobacterial cultures of freshwater and marine habitat, Spirulina, Phormidium and Lyngbya spp., were purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Blue-coloured crystals in different crystal forms, monoclinic and hexagonal, were obtained for the three species. The crystals took 1–12 weeks to grow to full size using polyethylene glycols of different molecular weights as precipitants. The amino-acid sequences of these proteins show high similarity to other known C-phycocyanins from related organisms; however, the C-phycocyanins reported here showed different biochemical and biophysical properties, i.e. molecular weight, stability etc. The X-ray diffraction data were collected at resolutions of 3.0 Å for the monoclinic and 3.2 and 3.6 Å for the hexagonal forms. The unit-cell parameters corresponding to the monoclinic space group P2{sub 1} are a = 107.33, b = 115.64, c = 183.26 Å, β = 90.03° for Spirulina sp. C-phycocyanin and are similar for crystals of Phormidium and Lyngbya spp. C-phycocyanins. Crystals belonging to the hexagonal space group P6{sub 3}, with unit-cell parameters a = b = 154.97, c = 40.35 Å and a = b = 151.96, c = 39.06 Å, were also obtained for the C-phycocyanins from Spirulina and Lyngbya spp., respectively. The estimated solvent content is around 50% for the monoclinic crystals of all three species assuming the presence of two hexamers per asymmetric unit. The solvent content is 66.5 and 64.1% for the hexagonal crystals of C-phycocyanin from Spirulina and Lyngbya spp. assuming the presence of one αβ monomer per asymmetric unit.},
doi = {10.1107/S1744309105025649},
url = {https://www.osti.gov/biblio/22356156}, journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 9,
volume = 61,
place = {United Kingdom},
year = {Thu Sep 01 00:00:00 EDT 2005},
month = {Thu Sep 01 00:00:00 EDT 2005}
}