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Title: The rotational order–disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP

An analysis of the rotational order–disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP is presented. The rotational order–disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order–disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed.
Authors:
 [1] ;  [2] ; ;  [3] ;  [1]
  1. Argonne National Laboratory, Argonne, IL 60439 (United States)
  2. (United States)
  3. Albert Einstein College of Medicine, Bronx, NY 10461 (United States)
Publication Date:
OSTI Identifier:
22351301
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 70; Journal Issue: Pt 1; Other Information: PMCID: PMC3919260; PMID: 24419376; PUBLISHER-ID: yt5058; OAI: oai:pubmedcentral.nih.gov:3919260; Copyright (c) International Union of Crystallography 2014; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALS; DENSITY; DISTRIBUTION; ELECTRON DENSITY; ORIENTATION; PROTEINS; SYMMETRY