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Title: Dynamic characterization of hydrophobic and hydrophilic solutes in oleic-acid enhanced transdermal delivery using two-photon fluorescence microscopy

In this letter, we propose an efficient methodology of investigating dynamic properties of sulforhodamine B and rhodamine B hexyl ester molecules transporting across ex-vivo human stratum corneum with and without oleic acid enhancement. Three-dimensional, time-lapse fluorescence images of the stratum corneum can be obtained using two-photon fluorescence microscopy. Furthermore, temporal quantifications of transport enhancements in diffusion parameters can be achieved with the use of Fick's second law. Dynamic characterization of solutes transporting across the stratum corneum is an effective method for understanding transient phenomena in transdermal delivery of probe molecules, leading to improved delivery strategies of molecular species for therapeutic purposes.
Authors:
; ;  [1] ;  [2] ;  [1] ;  [3] ;  [3]
  1. Department of Physics, National Taiwan University, Taipei, Taiwan (China)
  2. Department of Dermatology, Far Eastern Memorial Hospital, New Taipei City, Taiwan (China)
  3. (China)
Publication Date:
OSTI Identifier:
22350995
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 16; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; DELIVERY; DIFFUSION; FLUORESCENCE; IMAGES; MICROSCOPY; MOLECULES; OLEIC ACID; PHOTONS; SOLUTES; THREE-DIMENSIONAL CALCULATIONS; TRANSIENTS