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Title: From lows to highs: using low-resolution models to phase X-ray data

An unusual example of how virus structure determination pushes the limits of the molecular replacement method is presented. The study of virus structures has contributed to methodological advances in structural biology that are generally applicable (molecular replacement and noncrystallographic symmetry are just two of the best known examples). Moreover, structural virology has been instrumental in forging the more general concept of exploiting phase information derived from multiple structural techniques. This hybridization of structural methods, primarily electron microscopy (EM) and X-ray crystallography, but also small-angle X-ray scattering (SAXS) and nuclear magnetic resonance (NMR) spectroscopy, is central to integrative structural biology. Here, the interplay of X-ray crystallography and EM is illustrated through the example of the structural determination of the marine lipid-containing bacteriophage PM2. Molecular replacement starting from an ∼13 Å cryo-EM reconstruction, followed by cycling density averaging, phase extension and solvent flattening, gave the X-ray structure of the intact virus at 7 Å resolution This in turn served as a bridge to phase, to 2.5 Å resolution, data from twinned crystals of the major coat protein (P2), ultimately yielding a quasi-atomic model of the particle, which provided significant insights into virus evolution and viral membrane biogenesis.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [2]
  1. University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN (United Kingdom)
  2. (United Kingdom)
  3. CIC bioGUNE, CIBERehd, Bizkaia Technology Park, Bld 800, 48160 Derio (Spain)
  4. (Spain)
Publication Date:
OSTI Identifier:
22347838
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 69; Journal Issue: Pt 11; Other Information: PMCID: PMC3817700; PMID: 24189238; PUBLISHER-ID: ba5207; OAI: oai:pubmedcentral.nih.gov:3817700; Copyright (c) Stuart & Abrescia 2013; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ATOMS; CRYSTALLOGRAPHY; CRYSTALS; DENSITY; ELECTRON MICROSCOPY; ELECTRONS; EVOLUTION; MEMBRANES; NUCLEAR MAGNETIC RESONANCE; PROTEINS; RESOLUTION; SCATTERING; SPECTROSCOPY; SYMMETRY