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Title: In cellulo structure determination of a novel cypovirus polyhedrin

The crystal structure of a previously unsolved type of cypovirus polyhedrin has been determined from data collected directly from frozen live insect cells. This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [2] ;  [3]
  1. Diamond Light Source Ltd, Harwell Oxford, Didcot OX11 0DE (United Kingdom)
  2. University of Oxford, Oxford OX3 7BN (United Kingdom)
  3. (United Kingdom)
Publication Date:
OSTI Identifier:
22347799
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 70; Journal Issue: Pt 5; Other Information: PMCID: PMC4014125; PMID: 24816111; PUBLISHER-ID: wa5065; OAI: oai:pubmedcentral.nih.gov:4014125; Copyright (c) Axford et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BEAMS; CRYSTAL STRUCTURE; CRYSTALS; ENVIRONMENT; IRON; SYNCHROTRONS