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Title: Neutron scattering from myelin revisited: bilayer asymmetry and water-exchange kinetics

The structure of internodal myelin in the rodent central and peripheral nervous systems has been determined using neutron diffraction. The kinetics of water exchange in these tissues is also described. Rapid nerve conduction in the central and peripheral nervous systems (CNS and PNS, respectively) of higher vertebrates is brought about by the ensheathment of axons with myelin, a lipid-rich, multilamellar assembly of membranes. The ability of myelin to electrically insulate depends on the regular stacking of these plasma membranes and on the presence of a number of specialized membrane-protein assemblies in the sheath, including the radial component, Schmidt–Lanterman incisures and the axo–glial junctions of the paranodal loops. The disruption of this fine-structure is the basis for many demyelinating neuropathies in the CNS and PNS. Understanding the processes that govern myelin biogenesis, maintenance and destabilization requires knowledge of myelin structure; however, the tight packing of internodal myelin and the complexity of its junctional specializations make myelin a challenging target for comprehensive structural analysis. This paper describes an examination of myelin from the CNS and PNS using neutron diffraction. This investigation revealed the dimensions of the bilayers and aqueous spaces of myelin, asymmetry between the cytoplasmic and extracellular leaflets of the membrane,more » and the distribution of water and exchangeable hydrogen in internodal multilamellar myelin. It also uncovered differences between CNS and PNS myelin in their water-exchange kinetics.« less
Authors:
 [1] ; ;  [2] ;  [3] ;  [4] ;  [1]
  1. Boston College, Chestnut Hill, MA 02467 (United States)
  2. Institut Laue–Langevin (ILL), CS 20156, F-38042 Grenoble CEDEX 9 (France)
  3. European Synchrotron Radiation Facility (ESRF), CS 40220, F-38043 Grenoble CEDEX 9 (France)
  4. NOVA Scientific Inc., Sturbridge, MA 01566 (United States)
Publication Date:
OSTI Identifier:
22347730
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 70; Journal Issue: Pt 12; Other Information: PMCID: PMC4257619; PMID: 25478838; PUBLISHER-ID: mn5073; OAI: oai:pubmedcentral.nih.gov:4257619; Copyright (c) Denninger et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ASYMMETRY; DISTRIBUTION; FINE STRUCTURE; HYDROGEN; KINETICS; LAYERS; NEUTRON DIFFRACTION; PLASMA; STOWING; SYMMETRY; WATER