Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2, 4-pentanediol
- Yeshiva University, 2495 Amsterdam Avenue, New York, NY 10033-3312 (United States)
- Brookhaven National Laboratory, Building 725D, Upton, NY 11973-5000 (United States)
- Collegiate School, 260 West 78th Street, New York, NY 10024-6559 (United States)
Crystallization of lysozyme with (R)-2-methyl-2, 4-pentanediol produces more ordered crystals and a higher resolution protein structure than crystallization with (S)-2-methyl-2, 4-pentanediol. The results suggest that chiral interactions with chiral additives are important in protein crystal formation. Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2, 4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.
- OSTI ID:
- 22347717
- Journal Information:
- Acta Crystallographica. Section D: Biological Crystallography, Vol. 71, Issue Pt 3; Other Information: PMCID: PMC4356360; PMID: 25760593; PUBLISHER-ID: nj5208; OAI: oai:pubmedcentral.nih.gov:4356360; Copyright (c) Stauber et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0907-4449
- Country of Publication:
- Denmark
- Language:
- English
An Analysis on Commercial Screening Kits and Chemical Components in Biomacromolecular Crystallization Screening
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journal | August 2019 |
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