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Title: Characterization and antibacterial properties of stable silver substituted hydroxyapatite nanoparticles synthesized through surfactant assisted microwave process

Highlights: • Stable nano sized silver substitute hydroxyapatite is prepared under surfactant assisted microwave process at 600 W power for 7 min. • The nanoparticles are in the size range of 58–72 nm and exert uniform elongated spheroid morphology. • Increase in silver concentration resulted in better dielectric properties. • Good antibacterial activity and silver release. - Abstract: The present study reports a relatively simple method for the synthesis of stable silver substituted hydroxyapatite nanoparticles with controlled morphology and particle size. In order to achieve this, CTAB is included as a surfactant in the microwave refluxing process (600 W for 7 min). The nanoparticles produced with different silver ion concentrations (0.05, 0.1 and 0.2 wt%) were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscope (FESEM), energy dispersive X-ray (EDX) and Brunauer–Emmett–Teller (BET) analysis. XRD and FTIR analyses reveal that the Ag-HA nanoparticles were phase pure at 1000 °C. FESEM images showed that the produced nanoparticles are in the size range of 58–72 nm and exert uniform elongated spheroid morphology. The dielectric properties suggest that the increase in dielectric constant (ε′) and dissipation factor (D) values with increasing Ag concentrations. Antibacterial performance of themore » Ag-HA samples elucidated using disk diffusion technique (DDT) and minimum inhibitory concentration (MIC) demonstrates anti-bacterial activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. This effect was dose dependent and was more pronounced against Gram-negative bacteria than Gram-positive organisms.« less
Authors:
 [1] ;  [1] ;  [2] ;  [1] ; ;  [3]
  1. Medical Implant Technology Group (MEDITEG), Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor Darul Takzim (Malaysia)
  2. Faculty of Bioscience and Medical Engineering (FBME), Universiti Teknologi Malaysia, 81310 Skudai, Johor Darul Takzim (Malaysia)
  3. Tissue Engineering Group, NOCERAL, Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur (Malaysia)
Publication Date:
OSTI Identifier:
22341763
Resource Type:
Journal Article
Resource Relation:
Journal Name: Materials Research Bulletin; Journal Volume: 48; Journal Issue: 9; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY; APATITES; BACILLUS SUBTILIS; CERAMICS; CRYSTAL STRUCTURE; ESCHERICHIA COLI; FIELD EMISSION; FOURIER TRANSFORMATION; INFRARED SPECTRA; NANOPARTICLES; NANOSTRUCTURES; PARTICLE SIZE; PERMITTIVITY; PSEUDOMONAS; SCANNING ELECTRON MICROSCOPY; STAPHYLOCOCCUS; SURFACTANTS; SYNTHESIS; X RADIATION; X-RAY DIFFRACTION