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Title: Associations between bacterial communities of house dust and infant gut

The human gut is host to a diverse and abundant community of bacteria that influence health and disease susceptibility. This community develops in infancy, and its composition is strongly influenced by environmental factors, notably perinatal anthropogenic exposures such as delivery mode (Cesarean vs. vaginal) and feeding method (breast vs. formula); however, the built environment as a possible source of exposure has not been considered. Here we report on a preliminary investigation of the associations between bacteria in house dust and the nascent fecal microbiota from 20 subjects from the Canadian Healthy Infant Longitudinal Development (CHILD) Study using high-throughput sequence analysis of portions of the 16S rRNA gene. Despite significant differences between the dust and fecal microbiota revealed by Nonmetric Multidimensional Scaling (NMDS) analysis, permutation analysis confirmed that 14 bacterial OTUs representing the classes Actinobacteria (3), Bacilli (3), Clostridia (6) and Gammaproteobacteria (2) co-occurred at a significantly higher frequency in matched dust–stool pairs than in randomly permuted pairs, indicating an association between these dust and stool communities. These associations could indicate a role for the indoor environment in shaping the nascent gut microbiota, but future studies will be needed to confirm that our findings do not solely reflect a reverse pathway.more » Although pet ownership was strongly associated with the presence of certain genera in the dust for dogs (Agrococcus, Carnobacterium, Exiguobacterium, Herbaspirillum, Leifsonia and Neisseria) and cats (Escherichia), no clear patterns were observed in the NMDS-resolved stool community profiles as a function of pet ownership.« less
Authors:
;  [1] ;  [2] ;  [1] ;  [3] ;  [2] ;  [4] ;  [5] ;  [1] ;  [6] ;  [7] ;  [3] ;  [1]
  1. Division of Occupational and Environmental Health, Dalla Lana School of Public Health, University of Toronto (Canada)
  2. Department of Cell and Systems Biology, University of Toronto (Canada)
  3. Department of Pediatrics, University of Alberta (Canada)
  4. Department of Medicine, McMaster University (Canada)
  5. University of Manitoba (Canada)
  6. (Canada)
  7. Faculty of Health Science, Simon Fraser University (Canada)
Publication Date:
OSTI Identifier:
22337282
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Research; Journal Volume: 131; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BACTERIA; CATS; DOGS; DUSTS; GENES; INDOOR AIR POLLUTION; INFANTS; MAMMARY GLANDS; RNA; STRUCTURAL CHEMICAL ANALYSIS