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Title: In vivo, label-free, three-dimensional quantitative imaging of liver surface using multi-photon microscopy

Various structural features on the liver surface reflect functional changes in the liver. The visualization of these surface features with molecular specificity is of particular relevance to understanding the physiology and diseases of the liver. Using multi-photon microscopy (MPM), we have developed a label-free, three-dimensional quantitative and sensitive method to visualize various structural features of liver surface in living rat. MPM could quantitatively image the microstructural features of liver surface with respect to the sinuosity of collagen fiber, the elastic fiber structure, the ratio between elastin and collagen, collagen content, and the metabolic state of the hepatocytes that are correlative with the pathophysiologically induced changes in the regions of interest. This study highlights the potential of this technique as a useful tool for pathophysiological studies and possible diagnosis of the liver diseases with further development.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [3] ;  [1] ;  [1] ;  [3] ;  [3] ;  [4] ;  [3] ;  [3] ;
  1. Biosystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore (Singapore)
  2. (China)
  3. (Singapore)
  4. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore (Singapore)
Publication Date:
OSTI Identifier:
22311111
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 2; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; COLLAGEN; COMPUTERIZED SIMULATION; DIAGNOSIS; DISEASES; FIBERS; IMAGES; IN VIVO; LIVER; LIVER CELLS; MICROSCOPY; MICROSTRUCTURE; MULTI-PHOTON PROCESSES; SPECIFICITY; SURFACES; THREE-DIMENSIONAL CALCULATIONS; TOOLS