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Title: In vivo, label-free, three-dimensional quantitative imaging of liver surface using multi-photon microscopy

Abstract

Various structural features on the liver surface reflect functional changes in the liver. The visualization of these surface features with molecular specificity is of particular relevance to understanding the physiology and diseases of the liver. Using multi-photon microscopy (MPM), we have developed a label-free, three-dimensional quantitative and sensitive method to visualize various structural features of liver surface in living rat. MPM could quantitatively image the microstructural features of liver surface with respect to the sinuosity of collagen fiber, the elastic fiber structure, the ratio between elastin and collagen, collagen content, and the metabolic state of the hepatocytes that are correlative with the pathophysiologically induced changes in the regions of interest. This study highlights the potential of this technique as a useful tool for pathophysiological studies and possible diagnosis of the liver diseases with further development.

Authors:
 [1];  [1];  [1];  [1];  [2];
  1. Biosystems and Micromechanics IRG, Singapore-MIT Alliance for Research and Technology, 1 CREATE Way, #04-13/14 Enterprise Wing, 138602 Singapore (Singapore)
  2. Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore (Singapore)
Publication Date:
OSTI Identifier:
22311111
Resource Type:
Journal Article
Journal Name:
Applied Physics Letters
Additional Journal Information:
Journal Volume: 105; Journal Issue: 2; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0003-6951
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; COLLAGEN; COMPUTERIZED SIMULATION; DIAGNOSIS; DISEASES; FIBERS; IMAGES; IN VIVO; LIVER; LIVER CELLS; MICROSCOPY; MICROSTRUCTURE; MULTI-PHOTON PROCESSES; SPECIFICITY; SURFACES; THREE-DIMENSIONAL CALCULATIONS; TOOLS

Citation Formats

Zhuo, Shuangmu, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, Yan, Jie, Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, MD 11 #04-01A, 117599 Singapore, Kang, Yuzhan, Xu, Shuoyu, Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore, Peng, Qiwen, Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore, Mechanobiology Institute, 5A Engineering Drive 1, T-Lab #05-01, 117411 Singapore, and others, and. In vivo, label-free, three-dimensional quantitative imaging of liver surface using multi-photon microscopy. United States: N. p., 2014. Web. doi:10.1063/1.4890593.
Zhuo, Shuangmu, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, Yan, Jie, Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, MD 11 #04-01A, 117599 Singapore, Kang, Yuzhan, Xu, Shuoyu, Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore, Peng, Qiwen, Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore, Mechanobiology Institute, 5A Engineering Drive 1, T-Lab #05-01, 117411 Singapore, & others, and. In vivo, label-free, three-dimensional quantitative imaging of liver surface using multi-photon microscopy. United States. https://doi.org/10.1063/1.4890593
Zhuo, Shuangmu, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, Yan, Jie, Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, MD 11 #04-01A, 117599 Singapore, Kang, Yuzhan, Xu, Shuoyu, Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore, Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore, Peng, Qiwen, Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore, Mechanobiology Institute, 5A Engineering Drive 1, T-Lab #05-01, 117411 Singapore, and others, and. 2014. "In vivo, label-free, three-dimensional quantitative imaging of liver surface using multi-photon microscopy". United States. https://doi.org/10.1063/1.4890593.
@article{osti_22311111,
title = {In vivo, label-free, three-dimensional quantitative imaging of liver surface using multi-photon microscopy},
author = {Zhuo, Shuangmu and Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007 and Yan, Jie and Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore and Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, MD 11 #04-01A, 117599 Singapore and Kang, Yuzhan and Xu, Shuoyu and Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, #04-01, 138669 Singapore and Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore and Peng, Qiwen and Computation and System Biology Program, Singapore-MIT Alliance, 4 Engineering Drive 3, E4-04-10, 117576 Singapore and Mechanobiology Institute, 5A Engineering Drive 1, T-Lab #05-01, 117411 Singapore and others, and},
abstractNote = {Various structural features on the liver surface reflect functional changes in the liver. The visualization of these surface features with molecular specificity is of particular relevance to understanding the physiology and diseases of the liver. Using multi-photon microscopy (MPM), we have developed a label-free, three-dimensional quantitative and sensitive method to visualize various structural features of liver surface in living rat. MPM could quantitatively image the microstructural features of liver surface with respect to the sinuosity of collagen fiber, the elastic fiber structure, the ratio between elastin and collagen, collagen content, and the metabolic state of the hepatocytes that are correlative with the pathophysiologically induced changes in the regions of interest. This study highlights the potential of this technique as a useful tool for pathophysiological studies and possible diagnosis of the liver diseases with further development.},
doi = {10.1063/1.4890593},
url = {https://www.osti.gov/biblio/22311111}, journal = {Applied Physics Letters},
issn = {0003-6951},
number = 2,
volume = 105,
place = {United States},
year = {Mon Jul 14 00:00:00 EDT 2014},
month = {Mon Jul 14 00:00:00 EDT 2014}
}