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Title: Evidence of radicals created by plasma in bacteria in water

Heavy water (D{sub 2}O) was introduced into a non-thermal plasma-jet (NTPJ) device to generate deuterium monoxide (OD) radicals at room temperature. Owing to the similar reactivity and low prevalence of deuterium in nature, OD radicals can be utilized to visualize the OH radical interactions with water and living cells. Escherichia coli in water were treated with OD radicals, and D atom incorporation into cells was visualized using time-of-flight SIMS and Nano-SIMS. The results show that D atoms from NTPJ reach the cytoplasm of E. coli in H{sub 2}O, indicating the usefulness of this OD-tracking method for the study of radical interactions with living cells.
Authors:
 [1] ;  [2] ;  [3] ; ; ; ;  [1] ;  [4]
  1. Department of Plasma Bioscience and Display, Kwangwoon University, 20 Kwangwoon-gil, Nowon-gu, Seoul 139-701 (Korea, Republic of)
  2. Department of Electrical and Biological Physics, Kwangwoon University, 20 Kwangwoon-gil, Nowon-gu, Seoul 139-701 (Korea, Republic of)
  3. High Technology Components and Materials Research Center, Korea Basic Science Institute, 1275 Jisa-dong, Gangseo-gu, Busan 618-230 (Korea, Republic of)
  4. (Korea, Republic of)
Publication Date:
OSTI Identifier:
22310924
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 7; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BIOLOGICAL EFFECTS; CYTOPLASM; DEUTERIUM; ESCHERICHIA COLI; HEAVY WATER; HYDROXYL RADICALS; INTERACTIONS; ION MICROPROBE ANALYSIS; MASS SPECTROSCOPY; PLASMA JETS; REACTIVITY; TEMPERATURE RANGE 0273-0400 K; TIME-OF-FLIGHT METHOD