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Title: Copper oxide assisted cysteine hierarchical structures for immunosensor application

The present work describes the promising electrochemical immunosensing strategy based on copper (II) assisted hierarchical cysteine structures (CuCys) varying from star to flower like morphology. The CuCys having average size of 10 μm have been synthesised using L-Cysteine as initial precursor in presence of copper oxide under environmentally friendly conditions in aqueous medium. To delineate the synthesis mechanism, detailed structural investigations have been carried out using characterization techniques such as X-ray diffraction, transmission electron microscopy, and Fourier transform infrared spectroscopy. The electrochemical behaviour of self-assembled CuCys on gold electrode shows surface controlled electrode reaction with an apparent electron transfer rate constant of 3.38 × 10{sup −4 }cm s{sup −1}. This innovative platform has been utilized to fabricate an immunosensor by covalently immobilizing monoclonal antibodies specific for Escherichia coli O157:H7 (E. coli). Under the optimal conditions, the fabricated immunosensor is found to be sensitive and specific for the detection of E. coli with a detection limit of 10 cfu/ml.
Authors:
 [1] ;  [2] ;  [1] ;  [3]
  1. Biomedical Instrumentation Section, CSIR-National Physical Laboratory, New Delhi 110012 (India)
  2. (India)
  3. Department of Chemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005 (India)
Publication Date:
OSTI Identifier:
22310856
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 10; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BIOLOGICAL EFFECTS; COPPER; COPPER OXIDES; COVALENCE; CYSTEINE; DETECTION; ELECTROCHEMISTRY; ELECTRODES; ELECTRON TRANSFER; ESCHERICHIA COLI; FOURIER TRANSFORM SPECTROMETERS; GOLD; MONOCLONAL ANTIBODIES; SENSITIVITY; SENSORS; SURFACES; SYNTHESIS; TRANSMISSION ELECTRON MICROSCOPY; X-RAY DIFFRACTION