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Title: Label-free three-dimensional imaging of cell nucleus using third-harmonic generation microscopy

We report the implementation of the combined third-harmonic generation (THG) and two-photon excited fluorescence (TPEF) microscopy for label-free three-dimensional (3-D) imaging of cell nucleus morphological changes in liver tissue. THG imaging shows regular spherical shapes of normal hepatocytes nuclei with inner chromatin structures while revealing the condensation of chromatins and nuclear fragmentations in hepatocytes of diseased liver tissue. Colocalized THG and TPEF imaging provides complementary information of cell nuclei and cytoplasm in tissue. This work suggests that 3-D THG microscopy has the potential for quantitative analysis of nuclear morphology in cells at a submicron-resolution without the need for DNA staining.
Authors:
; ; ;  [1]
  1. Optical Bioimaging Laboratory, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117576 (Singapore)
Publication Date:
OSTI Identifier:
22310855
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 10; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL NUCLEI; CHROMATIN; CYTOPLASM; DISEASES; DNA; FLUORESCENCE; HARMONIC GENERATION; IMAGES; LIVER; LIVER CELLS; MICROSCOPY; MORPHOLOGICAL CHANGES; NUCLEAR FRAGMENTATION; PLANT TISSUES; RESOLUTION; SPHERICAL CONFIGURATION; STAINS; THREE-DIMENSIONAL CALCULATIONS