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Title: Fabrication of optical multilayer for two-color phase plate in super-resolution microscope

In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.
Authors:
 [1] ;  [2] ; ; ;  [3] ;  [4]
  1. Olympus Corporation, 2-3 Kuboyama-cho, Hachioji 192-8512 (Japan)
  2. Toray Engineering Co. Ltd., 1-1-45 Oe, Otsu 520-2141 (Japan)
  3. Ceratech Japan Co., Ltd., 500 Okada, Shinonoi, Nagano 381-2295 (Japan)
  4. Department of Physics, Budapest University of Technology and Economics, Budapest 1111 (Hungary)
Publication Date:
OSTI Identifier:
22308826
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 85; Journal Issue: 7; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; BEAM OPTICS; BEAM PROFILES; BEAMS; COLOR; DESIGN; FABRICATION; FLUORESCENCE; INTERFEROMETRY; LAYERS; MICROSCOPES; MICROSCOPY; OPTIMIZATION; PHASE SHIFT; RESOLUTION