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Title: Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen–vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser–fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.
Authors:
;  [1] ;  [2] ;  [2] ; ;  [1] ;  [2] ;  [3] ;  [2] ;  [4] ;  [5] ;  [1] ;  [2] ;  [6] ;  [2]
  1. International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation)
  2. (Russian Federation)
  3. Kurchatov Institute National Research Center, Moscow (Russian Federation)
  4. B.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, Minsk (Belarus)
  5. National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044 (Japan)
  6. (United States)
Publication Date:
OSTI Identifier:
22293087
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 104; Journal Issue: 8; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; BRAIN; EXCITATION; FIBER OPTICS; FLUORESCENCE; LASERS; LOCALITY; MICE; MODE LOCKING; MULTI-PHOTON PROCESSES; NERVE TISSUE; NITROGEN; PHOTONS; SCATTERING; VACANCIES; YTTERBIUM