skip to main content

Title: Biodiesel versus diesel exposure: Enhanced pulmonary inflammation, oxidative stress, and differential morphological changes in the mouse lung

The use of biodiesel (BD) or its blends with petroleum diesel (D) is considered to be a viable approach to reduce occupational and environmental exposures to particulate matter (PM). Due to its lower particulate mass emissions compared to D, use of BD is thought to alleviate adverse health effects. Considering BD fuel is mainly composed of unsaturated fatty acids, we hypothesize that BD exhaust particles could induce pronounced adverse outcomes, due to their ability to readily oxidize. The main objective of this study was to compare the effects of particles generated by engine fueled with neat BD and neat petroleum-based D. Biomarkers of tissue damage and inflammation were significantly elevated in lungs of mice exposed to BD particulates. Additionally, BD particulates caused a significant accumulation of oxidatively modified proteins and an increase in 4-hydroxynonenal. The up-regulation of inflammatory cytokines/chemokines/growth factors was higher in lungs upon BD particulate exposure. Histological evaluation of lung sections indicated presence of lymphocytic infiltrate and impaired clearance with prolonged retention of BD particulate in pigment laden macrophages. Taken together, these results clearly indicate that BD exhaust particles could exert more toxic effects compared to D. - Highlights: • Exposure of mice to BDPM caused higher pulmonarymore » toxicity compared to DPM. • Oxidative stress and inflammation were higher in BD vs to D exposed mice. • Inflammatory lymphocyte infiltrates were seen only in lungs of mice exposed to BD. • Ineffective clearance, prolonged PM retention was present only after BD exposure.« less
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [4] ;  [3] ;  [1] ;  [5] ;  [2] ;  [1] ;  [6]
  1. Pathology and Physiology Research Branch/NIOSH/CDC, Morgantown, WV 26505 (United States)
  2. Office of Mine Safety and Health Research/NIOSH/CDC, Pittsburgh, PA 15236 (United States)
  3. Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA (United States)
  4. NIOSH/CDC, 4676 Columbia Parkway, Cincinnati, OH 45226 (United States)
  5. Department of Environmental and Occupational Health, University of Pittsburgh, PA (United States)
  6. (United States)
Publication Date:
OSTI Identifier:
22285430
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 272; Journal Issue: 2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIODIESEL FUELS; BIOLOGICAL MARKERS; CARBOXYLIC ACIDS; COMPARATIVE EVALUATIONS; DROPLETS; ENVIRONMENTAL EXPOSURE; INFLAMMATION; LUNGS; LYMPHOCYTES; LYMPHOKINES; MACROPHAGES; MICE; MORPHOLOGICAL CHANGES; OXIDATION; PARTICULATES; PETROLEUM; STRESSES; TOXICITY