Enzyme sequence similarity improves the reaction alignment method for cross-species pathway comparison
Abstract
Pathway-based information has become an important source of information for both establishing evolutionary relationships and understanding the mode of action of a chemical or pharmaceutical among species. Cross-species comparison of pathways can address two broad questions: comparison in order to inform evolutionary relationships and to extrapolate species differences used in a number of different applications including drug and toxicity testing. Cross-species comparison of metabolic pathways is complex as there are multiple features of a pathway that can be modeled and compared. Among the various methods that have been proposed, reaction alignment has emerged as the most successful at predicting phylogenetic relationships based on NCBI taxonomy. We propose an improvement of the reaction alignment method by accounting for sequence similarity in addition to reaction alignment method. Using nine species, including human and some model organisms and test species, we evaluate the standard and improved comparison methods by analyzing glycolysis and citrate cycle pathways conservation. In addition, we demonstrate how organism comparison can be conducted by accounting for the cumulative information retrieved from nine pathways in central metabolism as well as a more complete study involving 36 pathways common in all nine species. Our results indicate that reaction alignment with enzyme sequencemore »
- Authors:
-
- Chemical and Biochemical Engineering Department, Rutgers University, Piscataway, NJ 08854 (United States)
- Publication Date:
- OSTI Identifier:
- 22285385
- Resource Type:
- Journal Article
- Journal Name:
- Toxicology and Applied Pharmacology
- Additional Journal Information:
- Journal Volume: 271; Journal Issue: 3; Other Information: Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; BIOLOGICAL PATHWAYS; COMPARATIVE EVALUATIONS; DRUGS; ENZYMES; GENE REGULATION; GLYCOLYSIS; PSEUDOMONAS; TOXICITY
Citation Formats
Ovacik, Meric A., Androulakis, Ioannis P., E-mail: yannis@rci.rutgers.edu, and Biomedical Engineering Department, Rutgers University, Piscataway, NJ 08854. Enzyme sequence similarity improves the reaction alignment method for cross-species pathway comparison. United States: N. p., 2013.
Web. doi:10.1016/J.TAAP.2010.09.009.
Ovacik, Meric A., Androulakis, Ioannis P., E-mail: yannis@rci.rutgers.edu, & Biomedical Engineering Department, Rutgers University, Piscataway, NJ 08854. Enzyme sequence similarity improves the reaction alignment method for cross-species pathway comparison. United States. https://doi.org/10.1016/J.TAAP.2010.09.009
Ovacik, Meric A., Androulakis, Ioannis P., E-mail: yannis@rci.rutgers.edu, and Biomedical Engineering Department, Rutgers University, Piscataway, NJ 08854. 2013.
"Enzyme sequence similarity improves the reaction alignment method for cross-species pathway comparison". United States. https://doi.org/10.1016/J.TAAP.2010.09.009.
@article{osti_22285385,
title = {Enzyme sequence similarity improves the reaction alignment method for cross-species pathway comparison},
author = {Ovacik, Meric A. and Androulakis, Ioannis P., E-mail: yannis@rci.rutgers.edu and Biomedical Engineering Department, Rutgers University, Piscataway, NJ 08854},
abstractNote = {Pathway-based information has become an important source of information for both establishing evolutionary relationships and understanding the mode of action of a chemical or pharmaceutical among species. Cross-species comparison of pathways can address two broad questions: comparison in order to inform evolutionary relationships and to extrapolate species differences used in a number of different applications including drug and toxicity testing. Cross-species comparison of metabolic pathways is complex as there are multiple features of a pathway that can be modeled and compared. Among the various methods that have been proposed, reaction alignment has emerged as the most successful at predicting phylogenetic relationships based on NCBI taxonomy. We propose an improvement of the reaction alignment method by accounting for sequence similarity in addition to reaction alignment method. Using nine species, including human and some model organisms and test species, we evaluate the standard and improved comparison methods by analyzing glycolysis and citrate cycle pathways conservation. In addition, we demonstrate how organism comparison can be conducted by accounting for the cumulative information retrieved from nine pathways in central metabolism as well as a more complete study involving 36 pathways common in all nine species. Our results indicate that reaction alignment with enzyme sequence similarity results in a more accurate representation of pathway specific cross-species similarities and differences based on NCBI taxonomy.},
doi = {10.1016/J.TAAP.2010.09.009},
url = {https://www.osti.gov/biblio/22285385},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 271,
place = {United States},
year = {Sun Sep 15 00:00:00 EDT 2013},
month = {Sun Sep 15 00:00:00 EDT 2013}
}