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Title: Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model

Abstract

Atopic dermatitis (AD) and allergic contact dermatitis (ACD) are common allergic and inflammatory skin diseases caused by a combination of eczema, scratching, pruritus, and cutaneous sensitization with allergens. This paper examines whether oleanolic acid acetate (OAA) modulates AD and ACD symptoms by using an existing AD model based on the repeated local exposure of mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene to the ears of BALB/c mice. In addition, the paper uses a 2,4-dinitrofluorobenzene-sensitized local lymph node assay (LLNA) for the ACD model. The oral administration of OAA over a four-week period attenuated AD symptoms in terms of decreased skin lesions, epidermal thickness, the infiltration of immune cells (CD4{sup +} cells, eosinophils, and mast cells), and serum IgE, IgG2a, and histamine levels. The gene expression of Th1, Th2, Th17, and Th22 cytokines was reduced by OAA in the lymph node and ear tissue, and the LLNA verified that OAA suppressed ACD. The oral administration of OAA over a three-day period attenuated ACD symptoms in terms of ear thickness, lymphocyte proliferation, and serum IgG2a levels. The gene expression of Th1, Th2, and Th17 cytokines was reduced by OAA in the thymus and ear tissue. Finally, to define the underlying mechanism,more » this paper uses a TNF-α/IFN-γ-activated human keratinocyte (HaCaT) model. OAA inhibited the expression of cytokines and chemokines through the downregulation of NF-κB and MAPKs in HaCaT cells. Taken together, the results indicate that OAA inhibited AD and ACD symptoms, suggesting that OAA may be effective in treating allergic skin disorders. - Highlights: • OAA reduced both acute and chronic AD symptoms. • OAA had a controlling effect on the immune reaction for ACD. • The effect of OAA on allergic skin disorders was comparable to the cyclosporine A. • OAA might be a candidate for the treatment of allergic skin disorders.« less

Authors:
 [1];  [2];  [1];  [3];  [4]; ;  [2];  [2];  [1]
  1. CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu 700-422 (Korea, Republic of)
  2. Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185 (Korea, Republic of)
  3. Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 700-412 (Korea, Republic of)
  4. School of Nano and Advanced Materials Science and Engineering, Gyeongsang National University, Jinju 660-701 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22285298
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 269; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACETATES; CYCLOSPORINE; DERMATITIS; ECZEMA; EOSINOPHILS; IMMUNE REACTIONS; INFLAMMATION; LYMPH NODES; LYMPHOKINES; MAST CELLS; MICE; SKIN

Citation Formats

Choi, Jin Kyeong, Oh, Hyun-Mee, Lee, Soyoung, Park, Jin-Woo, Khang, Dongwoo, Lee, Seung Woong, Lee, Woo Song, Rho, Mun-Chual, and Kim, Sang-Hyun. Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model. United States: N. p., 2013. Web. doi:10.1016/J.TAAP.2013.03.001.
Choi, Jin Kyeong, Oh, Hyun-Mee, Lee, Soyoung, Park, Jin-Woo, Khang, Dongwoo, Lee, Seung Woong, Lee, Woo Song, Rho, Mun-Chual, & Kim, Sang-Hyun. Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model. United States. https://doi.org/10.1016/J.TAAP.2013.03.001
Choi, Jin Kyeong, Oh, Hyun-Mee, Lee, Soyoung, Park, Jin-Woo, Khang, Dongwoo, Lee, Seung Woong, Lee, Woo Song, Rho, Mun-Chual, and Kim, Sang-Hyun. 2013. "Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model". United States. https://doi.org/10.1016/J.TAAP.2013.03.001.
@article{osti_22285298,
title = {Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model},
author = {Choi, Jin Kyeong and Oh, Hyun-Mee and Lee, Soyoung and Park, Jin-Woo and Khang, Dongwoo and Lee, Seung Woong and Lee, Woo Song and Rho, Mun-Chual and Kim, Sang-Hyun},
abstractNote = {Atopic dermatitis (AD) and allergic contact dermatitis (ACD) are common allergic and inflammatory skin diseases caused by a combination of eczema, scratching, pruritus, and cutaneous sensitization with allergens. This paper examines whether oleanolic acid acetate (OAA) modulates AD and ACD symptoms by using an existing AD model based on the repeated local exposure of mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene to the ears of BALB/c mice. In addition, the paper uses a 2,4-dinitrofluorobenzene-sensitized local lymph node assay (LLNA) for the ACD model. The oral administration of OAA over a four-week period attenuated AD symptoms in terms of decreased skin lesions, epidermal thickness, the infiltration of immune cells (CD4{sup +} cells, eosinophils, and mast cells), and serum IgE, IgG2a, and histamine levels. The gene expression of Th1, Th2, Th17, and Th22 cytokines was reduced by OAA in the lymph node and ear tissue, and the LLNA verified that OAA suppressed ACD. The oral administration of OAA over a three-day period attenuated ACD symptoms in terms of ear thickness, lymphocyte proliferation, and serum IgG2a levels. The gene expression of Th1, Th2, and Th17 cytokines was reduced by OAA in the thymus and ear tissue. Finally, to define the underlying mechanism, this paper uses a TNF-α/IFN-γ-activated human keratinocyte (HaCaT) model. OAA inhibited the expression of cytokines and chemokines through the downregulation of NF-κB and MAPKs in HaCaT cells. Taken together, the results indicate that OAA inhibited AD and ACD symptoms, suggesting that OAA may be effective in treating allergic skin disorders. - Highlights: • OAA reduced both acute and chronic AD symptoms. • OAA had a controlling effect on the immune reaction for ACD. • The effect of OAA on allergic skin disorders was comparable to the cyclosporine A. • OAA might be a candidate for the treatment of allergic skin disorders.},
doi = {10.1016/J.TAAP.2013.03.001},
url = {https://www.osti.gov/biblio/22285298}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 269,
place = {United States},
year = {Wed May 15 00:00:00 EDT 2013},
month = {Wed May 15 00:00:00 EDT 2013}
}