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Title: Size effect on transfection and cytotoxicity of nanoscale plasmid DNA/polyethyleneimine complexes for aerosol gene delivery

Nanoscale plasmid DNA (pDNA)/polyethyleneimine (PEI) complexes were fabricated in the aerosol state using a nebulization system consisting of a collison atomizer and a cool-walled diffusion dryer. The aerosol fabricated nanoscale complexes were collected and employed to determine fundamental properties of the complexes, such as size, structure, surface charge, and in vitro gene transfection efficiency and cytotoxicity. The results showed that mass ratio between pDNA and PEI should be optimized to enhance gene transfection efficiency without a significant loss of cell viability. These findings may support practical advancements in the field of nonviral gene delivery.
Authors:
 [1] ;  [2]
  1. Department of Chemistry, Purdue University, West Lafayette, Indiana 47907 (United States)
  2. Department of Digital Display Engineering, Hoseo University, Asan 336-795 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22283247
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 104; Journal Issue: 5; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
77 NANOSCIENCE AND NANOTECHNOLOGY; 60 APPLIED LIFE SCIENCES; AEROSOLS; DELIVERY; DIFFUSION; DNA; EFFICIENCY; GENES; IMINES; IN VITRO; NANOSTRUCTURES; ORGANIC POLYMERS; SURFACES; TOXICITY; VIABILITY