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Title: Cell migration or cytokinesis and proliferation? – Revisiting the “go or grow” hypothesis in cancer cells in vitro

The mortality of patients with solid tumors is mostly due to metastasis that relies on the interplay between migration and proliferation. The “go or grow” hypothesis postulates that migration and proliferation spatiotemporally excludes each other. We evaluated this hypothesis on 35 cell lines (12 mesothelioma, 13 melanoma and 10 lung cancer) on both the individual cell and population levels. Following three-day-long videomicroscopy, migration, proliferation and cytokinesis-length were quantified. We found a significantly higher migration in mesothelioma cells compared to melanoma and lung cancer while tumor types did not differ in mean proliferation or duration of cytokinesis. Strikingly, we found in melanoma and lung cancer a significant positive correlation between mean proliferation and migration. Furthermore, non-dividing melanoma and lung cancer cells displayed slower migration. In contrast, in mesothelioma there were no such correlations. Interestingly, negative correlation was found between cytokinesis-length and migration in melanoma. FAK activation was higher in melanoma cells with high motility. We demonstrate that the cancer cells studied do not defer proliferation for migration. Of note, tumor cells from various organ systems may differently regulate migration and proliferation. Furthermore, our data is in line with the observation of pathologists that highly proliferative tumors are often highly invasive. -more » Highlights: • We investigated the “go or grow” hypothesis in human cancer cells in vitro. • Proliferation and migration positively correlate in melanoma and lung cancer cells. • Duration of cytokinesis and migration shows inverse correlation. • Increased FAK activation is present in highly motile melanoma cells.« less
Authors:
; ;  [1] ;  [2] ;  [3] ;  [4] ; ; ;  [5] ;  [5] ;  [6] ;  [1] ;  [6] ;  [5] ;  [2] ;  [5] ;  [6]
  1. 2nd Department of Pathology, Semmelweis University, Budapest (Hungary)
  2. Institute of Cancer Research and Comprehensive Cancer Center, Medical University of Vienna, Vienna (Austria)
  3. Department of Biological Physics, Eötvös University, Budapest (Hungary)
  4. (United States)
  5. Department of Thoracic Surgery, Medical University of Vienna, Vienna (Austria)
  6. (Hungary)
Publication Date:
OSTI Identifier:
22278199
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 319; Journal Issue: 20; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; HYPOTHESIS; IN VITRO; LUNGS; MELANOMAS; METASTASES; MIGRATION; MORTALITY; TUMOR CELLS