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Title: Toward single cell traction microscopy within 3D collagen matrices

Mechanical interaction between the cell and its extracellular matrix (ECM) regulates cellular behaviors, including proliferation, differentiation, adhesion, and migration. Cells require the three-dimensional (3D) architectural support of the ECM to perform physiologically realistic functions. However, current understanding of cell–ECM and cell–cell mechanical interactions is largely derived from 2D cell traction force microscopy, in which cells are cultured on a flat substrate. 3D cell traction microscopy is emerging for mapping traction fields of single animal cells embedded in either synthetic or natively derived fibrous gels. We discuss here the development of 3D cell traction microscopy, its current limitations, and perspectives on the future of this technology. Emphasis is placed on strategies for applying 3D cell traction microscopy to individual tumor cell migration within collagen gels. - Highlights: • Review of the current state of the art in 3D cell traction force microscopy. • Bulk and micro-characterization of remodelable fibrous collagen gels. • Strategies for performing 3D cell traction microscopy within collagen gels.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [3] ;  [1]
  1. Department of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853 (United States)
  2. Department of Mechanical Engineering, University of Alberta, Edmonton, AB, Canada T6G 2G8 (Canada)
  3. Department of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853 (United States)
Publication Date:
OSTI Identifier:
22278170
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 319; Journal Issue: 16; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ATOMIC FORCE MICROSCOPY; COLLAGEN; FLUORESCENCE; GELS; POLYETHYLENE GLYCOLS; TUMOR CELLS