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Title: Diffusion studies on permeable nitroxyl spin probe through lipid bilayer membrane

Electron spin resonance (ESR) studies were carried out for 2mM {sup 14}N labeled deutrated permeable 3- methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water, 1 mM, 2 mM, 3 mM and 4 mM concentration of MC-PROXYL in 300 mM concentration of liposomal solution by using a L-band ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported. The partition parameter and permeability values indicate the maximum spin distribution in the lipid phase at 2 mM concentration. This study illustrates that ESR can be used to differentiate between the intra and extra-membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the radical concentration was optimized as 2 mM in liposomal solution for ESR phantom studies and experiments.
Authors:
;  [1] ; ; ;  [2] ;  [3] ;  [4]
  1. Department of Physics, NMSSVN College, Nagamalai, Madurai-625019 (India)
  2. Department of Bio-functional Science, Kyushu University, Fukuoka (Japan)
  3. Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka (Japan)
  4. Department of Chemistry, NMSSVN College, Nagamalai, Madurai-625 019 (India)
Publication Date:
OSTI Identifier:
22269427
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1591; Journal Issue: 1; Conference: 58. DAE solid state physics symposium 2013, Patiala, Punjab (India), 17-21 Dec 2013; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 36 MATERIALS SCIENCE; COUPLING CONSTANTS; DIFFUSION; ELECTRON SPIN RESONANCE; LANDE FACTOR; LAYERS; LIPIDS; MEMBRANES; NITROGEN 14; PARTITION; PERMEABILITY; PHANTOMS; SPIN; WATER