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Title: Effects of aqueous ammonia treatment on fiber’s surface morphology and enzymatic digestibility of empty fruit bunch fiber (EFBF)

This study was conducted to investigate the effects of aqueous ammonia reflux and soaked treatment on the fiber’s surface morphology and enzymatic digestibility of empty fruit bunch fiber (EFBF). The surface morphological changes of the fiber after aqueous ammonia treatment was linked to the sugars yield by enzymatic hydrolysis. The effectiveness of 6.25% aqueous ammonia treatment in improving enzymatic digestibility of EFBF was initially studied in reflux system and by soaking. The results showed that soaked treatment was more effective than reflux system. Further study on soaked treatment of EFBF was carried out by increasing the ammonia concentration to 12.50%. Soaking in aqueous ammonia was conducted at 30°C and 50°C for 24 hours. The results of enzymatic hydrolysis showed that sugar yield from EFBF soaked in 12.50% aqueous ammonia at 50°C was the highest. Approximately 242.91±15.50 mg/g EFBF of xylose and 320.49±28.31 mg/g EFBF of glucose were produced by the action of enzyme Cellic Ctec 2. Results of scanning electron microscopic showed that aqueous ammonia treatment by soaking had caused a more severe structural distortion on the fiber’s surface and higher removal of silica bodies that embedded on the fiber than those in reflux system. The changes on the fiber’smore » surface morphology were believed is the contributing factor that improved the enzymatic digestibility of EFBF after aqueous ammonia treatment.« less
Authors:
;  [1]
  1. Department of Food Science, School of Chemical Science and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi UKM, Selangor (Malaysia)
Publication Date:
OSTI Identifier:
22262656
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1571; Journal Issue: 1; Conference: 2013 UKM FST postgraduate colloquium, Selangor (Malaysia), 3-4 Jul 2013; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMMONIA; ENZYMATIC HYDROLYSIS; ENZYMES; FIBERS; FRUITS; GLUCOSE; SACCHAROSE; SCANNING ELECTRON MICROSCOPY; SILICA; SURFACES; XYLOSE