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Title: Self assembled monolayer based liquid crystal biosensor for free cholesterol detection

A unique cholesterol oxidase (ChOx) liquid crystal (LC) biosensor, based on the disruption of orientation in LCs, is developed for cholesterol detection. A self-assembled monolayer (SAM) of Dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP) and (3-Aminopropyl)trimethoxy-silane (APTMS) is prepared on a glass plate by adsorption. The enzyme (ChOx) is immobilized on SAM surface for 12 h before utilizing the film for biosensing purpose. LC based biosensing study is conducted on SAM/ChOx/LC (5CB) cells for cholesterol concentrations ranging from 10 mg/dl to 250 mg/dl. The sensing mechanism has been verified through polarizing optical microscopy, scanning electron microscopy, and spectrometric techniques.
Authors:
;  [1] ; ;  [2] ;  [3] ;  [1] ;  [4]
  1. Department of Science and Technology, Centre on Bimolecular Electronics, Biomedical Instrumentation Section, CSIR—National Physical Laboratory, Dr. K. S. Krishnan Road, New Delhi 110 012 (India)
  2. Polymeric and Soft Materials Section, CSIR—National Physical Laboratory (CSIR), Dr. K. S. Krishnan Road, New Delhi 110 012 (India)
  3. Centre for Physical and Mathematical Sciences, School of Basic and Applied Sciences, Central University of Punjab, City Campus, Mansa Road, Bathinda 151 001 (India)
  4. (CSIR), Dr. K. S. Krishnan Road, New Delhi 110 012 (India)
Publication Date:
OSTI Identifier:
22262626
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 104; Journal Issue: 15; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; AMMONIUM CHLORIDES; CHOLESTEROL; LIQUID CRYSTALS; SENSORS