skip to main content

SciTech ConnectSciTech Connect

Title: Cellular membrane collapse by atmospheric-pressure plasma jet

Cellular membrane dysfunction caused by air plasma in cancer cells has been studied to exploit atmospheric-pressure plasma jets for cancer therapy. Here, we report that plasma jet treatment of cervical cancer HeLa cells increased electrical conductivity across the cellular lipid membrane and caused simultaneous lipid oxidation and cellular membrane collapse. We made this finding by employing a self-manufactured microelectrode chip. Furthermore, increased roughness of the cellular lipid membrane and sequential collapse of the membrane were observed by atomic force microscopy following plasma jet treatment. These results suggest that the cellular membrane catastrophe occurs via coincident altered electrical conductivity, lipid oxidation, and membrane roughening caused by an atmospheric-pressure plasma jet, possibly resulting in cellular vulnerability to reactive species generated from the plasma as well as cytotoxicity to cancer cells.
Authors:
;  [1] ; ;  [2] ; ;  [3]
  1. Department of Electrical and Computer Engineering, Ajou University, Suwon 443-749 (Korea, Republic of)
  2. Department of Biological Sciences, Ajou University, Suwon 443-749 (Korea, Republic of)
  3. Department of Molecular Science and Technology, Ajou University, Suwon 443-749 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22257153
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 104; Journal Issue: 1; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ATOMIC FORCE MICROSCOPY; CELL MEMBRANES; ELECTRIC CONDUCTIVITY; HELA CELLS; LIPIDS