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Title: An optics-based variable-temperature assay system for characterizing thermodynamics of biomolecular reactions on solid support

A biological state is equilibrium of multiple concurrent biomolecular reactions. The relative importance of these reactions depends on physiological temperature typically between 10 °C and 50 °C. Experimentally the temperature dependence of binding reaction constants reveals thermodynamics and thus details of these biomolecular processes. We developed a variable-temperature opto-fluidic system for real-time measurement of multiple (400–10 000) biomolecular binding reactions on solid supports from 10 °C to 60 °C within ±0.1 °C. We illustrate the performance of this system with investigation of binding reactions of plant lectins (carbohydrate-binding proteins) with 24 synthetic glycans (i.e., carbohydrates). We found that the lectin-glycan reactions in general can be enthalpy-driven, entropy-driven, or both, and water molecules play critical roles in the thermodynamics of these reactions.
Authors:
; ;  [1] ; ; ; ; ; ;  [2]
  1. Department of Physics, University of California, One Shields Avenue, Davis, California 95616 (United States)
  2. Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616 (United States)
Publication Date:
OSTI Identifier:
22251550
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 84; Journal Issue: 11; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; CARBOHYDRATES; ENTHALPY; ENTROPY; LECTINS; MOLECULES; OPTICS; TEMPERATURE DEPENDENCE; THERMODYNAMICS; TIME MEASUREMENT