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Title: Reconfigurable acquisition system with integrated optics for a portable flow cytometer

Portable and inexpensive scientific instruments that are capable of performing point of care diagnostics are needed for applications such as disease detection and diagnosis in resource-poor settings, for water quality and food supply monitoring, and for biosurveillance activities in autonomous vehicles. In this paper, we describe the development of a compact flow cytometer built from three separate, customizable, and interchangeable modules. The instrument as configured in this work is being developed specifically for the detection of selected Centers for Disease Control (CDC) category B biothreat agents through a bead-based assay: E. coli O157:H7, Salmonella, Listeria, and Shigella. It has two-color excitation, three-color fluorescence and light scattering detection, embedded electronics, and capillary based flow. However, these attributes can be easily modified for other applications such as cluster of differentiation 4 (CD4) counting. Proof of concept is demonstrated through a 6-plex bead assay with the results compared to a commercially available benchtop-sized instrument.
Authors:
; ; ; ;  [1]
  1. Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375 (United States)
Publication Date:
OSTI Identifier:
22251397
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 84; Journal Issue: 11; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CAPILLARIES; COLOR MODEL; DETECTION; DIAGNOSIS; DISEASES; EXCITATION; FLUORESCENCE; FOOD; LIGHT SCATTERING; OPTICS; SALMONELLA; SHIGELLA; WATER QUALITY