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Title: A novel facility for 3D micro-irradiation of living cells in a controlled environment by MeV ions

We present a novel facility for micro-irradiation of living targets with ions from a 1.7 MV tandem accelerator. We show results using 1 MeV protons and 2 MeV He{sup 2+}. In contrast to common micro-irradiation facilities, which use electromagnetic or electrostatic focusing and specially designed vacuum windows, we employ a tapered glass capillary with a thin end window, made from polystyrene with a thickness of 1–2 μm, for ion focusing and extraction. The capillary is connected to a beamline tilted vertically by 45°, which allows for easy immersion of the extracted ions into liquid environment within a standard cell culture dish. An inverted microscope is used for simultaneously observing the samples as well as the capillary tip, while a stage-top incubator provides an appropriate environment for the samples. Furthermore, our setup allows to target volumes in cells within a μm{sup 3} resolution, while monitoring the target in real time during and after irradiation.
Authors:
; ; ; ; ; ; ;  [1] ; ;  [2]
  1. Atomic Physics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama (Japan)
  2. Cellular Dynamics Laboratory, RIKEN, 351-0198 Wako-shi, Saitama (Japan)
Publication Date:
OSTI Identifier:
22251198
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 85; Journal Issue: 1; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; 60 APPLIED LIFE SCIENCES; CAPILLARIES; CELL CULTURES; EXTRACTION; FOCUSING; HELIUM IONS; IRRADIATION; IRRADIATION PLANTS; MICROSCOPES; POLYSTYRENE; TANDEM ELECTROSTATIC ACCELERATORS; THICKNESS