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Title: Design and expression of a short peptide as an HIV detection probe

Abstract

Highlights: •We designed a short fusion peptide (FP-50) for in vivo expression. •This peptide is a very promising component for detection of gp120 protein. •The detectable level is about 20–200 times lower than previously published methods. •It is a novel probe to detect HIV-1 gp120 during early stages of HIV infection. -- Abstract: To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20–200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection.

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22242251
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 443; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AIDS VIRUS; AMINO ACIDS; ENZYME IMMUNOASSAY; ENZYMES; ESCHERICHIA COLI; FLUORESCEIN; IN VIVO; ISOTHIOCYANATES; MONOCLONAL ANTIBODIES; PEPTIDES

Citation Formats

Lines, Jamie A., Yu, Zhiqiang, Dedkova, Larisa M., and Chen, Shengxi. Design and expression of a short peptide as an HIV detection probe. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2013.11.095.
Lines, Jamie A., Yu, Zhiqiang, Dedkova, Larisa M., & Chen, Shengxi. Design and expression of a short peptide as an HIV detection probe. United States. https://doi.org/10.1016/J.BBRC.2013.11.095
Lines, Jamie A., Yu, Zhiqiang, Dedkova, Larisa M., and Chen, Shengxi. 2014. "Design and expression of a short peptide as an HIV detection probe". United States. https://doi.org/10.1016/J.BBRC.2013.11.095.
@article{osti_22242251,
title = {Design and expression of a short peptide as an HIV detection probe},
author = {Lines, Jamie A. and Yu, Zhiqiang and Dedkova, Larisa M. and Chen, Shengxi},
abstractNote = {Highlights: •We designed a short fusion peptide (FP-50) for in vivo expression. •This peptide is a very promising component for detection of gp120 protein. •The detectable level is about 20–200 times lower than previously published methods. •It is a novel probe to detect HIV-1 gp120 during early stages of HIV infection. -- Abstract: To explore a low-cost novel probe for HIV detection, we designed and prepared a 50-amino acid-length short fusion peptide (FP-50) via Escherichia coli in vivo expression. It was employed as a novel probe to detect HIV-1 gp120 protein. The detectable level of gp120 protein using the FP-50 peptide was approximately 20–200 times lower than previously published methods that used a pair of monoclonal antibodies. Thus, this short peptide is a very promising component for detection of gp120 protein during early stages of HIV infection.},
doi = {10.1016/J.BBRC.2013.11.095},
url = {https://www.osti.gov/biblio/22242251}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 443,
place = {United States},
year = {Fri Jan 03 00:00:00 EST 2014},
month = {Fri Jan 03 00:00:00 EST 2014}
}