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Title: Early postnatal maternal separation causes alterations in the expression of β3-adrenergic receptor in rat adipose tissue suggesting long-term influence on obesity

Highlights: •High-fat diet intake following maternal separation did not cause body weight gain. •However, levels of metabolism-related molecules in adipose tissue were altered. •Increased levels of prohibitin mRNA in white fat were observed. •Attenuated levels of β3-adrenergic receptor mRNA were observed in brown fat. •Such alterations in adipose tissue may contribute to obesity later in life. -- Abstract: The effects of early postnatal maternal deprivation on the biological characteristics of the adipose tissue later in life were investigated in the present study. Sprague–Dawley rats were classified as either maternal deprivation (MD) or mother-reared control (MRC) groups. MD was achieved by separating the rat pups from their mothers for 3 h each day during the 10–15 postnatal days. mRNA levels of mitochondrial uncoupling protein 1 (UCP-1), β3-adrenergic receptor (β3-AR), and prohibitin (PHB) in the brown and white adipose tissue were determined using real-time RT-PCR analysis. UCP-1, which is mediated through β3-AR, is closely involved in the energy metabolism and expenditure. PHB is highly expressed in the proliferating tissues/cells. At 10 weeks of age, the body weight of the MRC and MD rats was similar. However, the levels of the key molecules in the adipose tissue were substantially altered. There was amore » significant increase in the expression of PHB mRNA in the white adipose tissue, while the β3-AR mRNA expression decreased significantly, and the UCP-1 mRNA expression remained unchanged in the brown adipose tissue. Given that these molecules influence the mitochondrial metabolism, our study indicates that early postnatal maternal deprivation can influence the fate of adipose tissue proliferation, presumably leading to obesity later in life.« less
Authors:
 [1] ; ; ;  [1] ;  [2] ;  [1] ;  [3] ;  [4] ;  [5] ; ;  [1] ;  [6] ;  [7] ;  [1]
  1. Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University (Japan)
  2. Department of Pediatrics, Faculty of Medicine, Kagawa University (Japan)
  3. Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University (Japan)
  4. Department of Forensic Medicine, Faculty of Medicine, Kagawa University (Japan)
  5. Department of Anesthesia, Nishiwaki Municipal Hospital (Japan)
  6. Department of Medical Education, Faculty of Medicine, Kagawa University (Japan)
  7. Department of Clinical Neuroscience and Therapeutics, Hiroshima University Graduate School of Biomedical Sciences (Japan)
Publication Date:
OSTI Identifier:
22242223
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 442; Journal Issue: 1-2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; FATS; MESSENGER-RNA; METABOLIC DISEASES; METABOLISM; MITOCHONDRIA; MOTHS; POLYMERASE CHAIN REACTION; RATS; RECEPTORS