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Title: miRNA-205 affects infiltration and metastasis of breast cancer

Abstract

Highlights: •We detected expression of miR-205 in breast cancer cell lines and tissue samples. •We suggest miR-205 is downregulated in human breast cancer tissues and MCF7 cells. •We suggest the lower expression of miR-205 play a role in breast cancer onset. •These data suggest that miR-205 directly targets HER3 in human breast cancer. -- Abstract: Background: An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy. Methods: The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. Results: miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness.more » By bioinformation analysis, miR-205 was predicted to bind to the 3′ untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression. Conclusion: miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.« less

Authors:
 [1]; ; ; ; ;  [1];  [1]
  1. Department of Chest Surgery, The First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Xi’an 710061 (China)
Publication Date:
OSTI Identifier:
22242180
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 441; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; COLONY FORMATION; GROWTH FACTORS; INHIBITION; LUCIFERASE; MAMMARY GLANDS; MESSENGER-RNA; METASTASES; NEOPLASMS; POLYMERASE CHAIN REACTION; RECEPTORS

Citation Formats

Wang, Zhouquan, Department of Tumor, SenGong Hospital of Shaanxi, Xi’an 710300, Liao, Hehe, Deng, Zhiping, Yang, Po, Du, Ning, Zhanng, Yunfeng, and Ren, Hong. miRNA-205 affects infiltration and metastasis of breast cancer. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.10.025.
Wang, Zhouquan, Department of Tumor, SenGong Hospital of Shaanxi, Xi’an 710300, Liao, Hehe, Deng, Zhiping, Yang, Po, Du, Ning, Zhanng, Yunfeng, & Ren, Hong. miRNA-205 affects infiltration and metastasis of breast cancer. United States. https://doi.org/10.1016/J.BBRC.2013.10.025
Wang, Zhouquan, Department of Tumor, SenGong Hospital of Shaanxi, Xi’an 710300, Liao, Hehe, Deng, Zhiping, Yang, Po, Du, Ning, Zhanng, Yunfeng, and Ren, Hong. 2013. "miRNA-205 affects infiltration and metastasis of breast cancer". United States. https://doi.org/10.1016/J.BBRC.2013.10.025.
@article{osti_22242180,
title = {miRNA-205 affects infiltration and metastasis of breast cancer},
author = {Wang, Zhouquan and Department of Tumor, SenGong Hospital of Shaanxi, Xi’an 710300 and Liao, Hehe and Deng, Zhiping and Yang, Po and Du, Ning and Zhanng, Yunfeng and Ren, Hong},
abstractNote = {Highlights: •We detected expression of miR-205 in breast cancer cell lines and tissue samples. •We suggest miR-205 is downregulated in human breast cancer tissues and MCF7 cells. •We suggest the lower expression of miR-205 play a role in breast cancer onset. •These data suggest that miR-205 directly targets HER3 in human breast cancer. -- Abstract: Background: An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy. Methods: The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay. Results: miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness. By bioinformation analysis, miR-205 was predicted to bind to the 3′ untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression. Conclusion: miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.},
doi = {10.1016/J.BBRC.2013.10.025},
url = {https://www.osti.gov/biblio/22242180}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 441,
place = {United States},
year = {Fri Nov 08 00:00:00 EST 2013},
month = {Fri Nov 08 00:00:00 EST 2013}
}