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Title: Dual expression of hTERT and VEGF prolongs life span and enhances angiogenic ability of aged BMSCs

Highlights: •Expression of hTERT and VEGF changed the lifespan and morphology of hBMSCs. •The expression of VEGF and hTRET promoted angiogenesis in vitro and in vivo. •The expression of VEGF and hTRET in hBMSCs had few effects on tumorigenicity. -- Abstract: Previous studies have confirmed the therapeutic effects of bone marrow stromal cells (BMSCs) transplantation on cerebral ischemia. However, the proliferative, differentiative, and homing capacity of BMSC from the elderly are significantly reduced, especially after several passages expansion in vitro. In this study, by introducing lentivirus-mediated hTERT and VEGF genes to modify human BMSCs from aged donors, we observed extended lifespan, promoted angiogenic capacity while less enhanced tumorigenicity of the genetically engineering BMSCs. These results therefore suggest that the modification of aged BMSCs by dual expression of hTERT and VEGF may be used for autologous cell replacement for ischemic cerebrovascular disease in elderly patients.
Authors:
 [1] ;  [2] ;  [1] ;  [2] ; ; ; ; ; ; ; ; ;  [1] ;  [1] ;  [1] ;  [2]
  1. Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou (China)
  2. (China)
Publication Date:
OSTI Identifier:
22242157
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 440; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANGIOGENESIS; BONE MARROW; ENZYME IMMUNOASSAY; ENZYMES; FLUORESCENCE; GENETIC ENGINEERING; GROWTH FACTORS; IN VITRO; IN VIVO; ISCHEMIA; PATIENTS; RNA