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Title: Borna disease virus nucleoprotein inhibits type I interferon induction through the interferon regulatory factor 7 pathway

Highlights: •IRF7 nuclear localisation was inhibited by BDV persistently infected. •BDV N protein resistant to IFN induction both in BDV infected OL cell and N protein plasmid transfected OL cell. •BDV N protein is related to the inhibition of IRF7 nuclear localisation. -- Abstract: The expression of type I interferon (IFN) is one of the most potent innate defences against viral infection in higher vertebrates. Borna disease virus (BDV) establishes persistent, noncytolytic infections in animals and in cultured cells. Early studies have shown that the BDV phosphoprotein can inhibit the activation of type I IFN through the TBK1–IRF3 pathway. The function of the BDV nucleoprotein in the inhibition of IFN activity is not yet clear. In this study, we demonstrated IRF7 activation and increased IFN-α/β expression in a BDV-persistently infected human oligodendroglia cell line following RNA interference-mediated BDV nucleoprotein silencing. Furthermore, we showed that BDV nucleoprotein prevented the nuclear localisation of IRF7 and inhibited endogenous IFN induction by poly(I:C), coxsackie virus B3 and IFN-β. Our findings provide evidence for a previously undescribed mechanism by which the BDV nucleoprotein inhibits type I IFN expression by interfering with the IRF7 pathway.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [1] ; ;  [3] ;  [1] ; ; ;  [4] ;  [1] ;  [2] ;  [2]
  1. The Heilongjiang Key Laboratory of Immunity and Infection, Heilongjiang (China)
  2. (China)
  3. The Key Laboratory of Pathogenic Biology, Heilongjiang Higher Education Institutions (China)
  4. Department of Microbiology, Harbin Medical University (China)
Publication Date:
OSTI Identifier:
22242074
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 438; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL CULTURES; DISEASES; INDUCTION; INHIBITION; INTERFERON; PHOSPHOPROTEINS; RNA; VERTEBRATES; VIRUSES