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Title: Induction of cytoprotective autophagy in PC-12 cells by cadmium

Abstract

Highlights: •Cadmium can promote early upregulation of autophagy in PC-12 cells. •Autophagy precedes apoptosis in cadmium-treated PC-12 cells. •Cadmium-induced autophagy is cytoprotective in PC-12 cells. •Class III PI3K/beclin-1/Bcl-2 signaling pathway plays a positive role in cadmium-triggered autophagy. -- Abstract: Laboratory data have demonstrated that cadmium (Cd) may induce neuronal apoptosis. However, little is known about the role of autophagy in neurons. In this study, cell viability decreased in a dose- and time-dependent manner after treatment with Cd in PC-12 cells. As cells were exposed to Cd, the levels of LC3-II proteins became elevated, specific punctate distribution of endogenous LC3-II increased, and numerous autophagosomes appeared, which suggest that Cd induced a high level of autophagy. In the late stages of autophagy, an increase in the apoptosis ratio was observed. Likewise, pre-treatment with chloroquine (an autophagic inhibitor) and rapamycin (an autophagic inducer) resulted in an increased and decreased percentage of apoptosis in contrast to other Cd-treated groups, respectively. The results indicate that autophagy delayed apoptosis in Cd-treated PC-12 cells. Furthermore, co-treatment of cells with chloroquine reduced autophagy and cell activity. However, rapamycin had an opposite effect on autophagy and cell activity. Moreover, class III PI3 K/beclin-1/Bcl-2 signaling pathways served a function inmore » Cd-induced autophagy. The findings suggest that Cd can induce cytoprotective autophagy by activating class III PI3 K/beclin-1/Bcl-2 signaling pathways. In sum, this study strongly suggests that autophagy may serve a positive function in the reduction of Cd-induced cytotoxicity.« less

Authors:
 [1]; ; ; ; ; ; ; ;  [1];  [1]
  1. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009 (China)
Publication Date:
OSTI Identifier:
22242055
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 438; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; BORON CHLORIDES; CADMIUM; NERVE CELLS; PROTEINS; SIGNALS; TIME DEPENDENCE; TOXICITY; VIABILITY

Citation Formats

Wang, Qiwen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Bijie Pilot Area Research Institute of Bijie University, Bijie 551700, Zhu, Jiaqiao, Zhang, Kangbao, Jiang, Chenyang, Wang, Yi, Yuan, Yan, Bian, Jianchun, Liu, Xuezhong, Gu, Jianhong, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Liu, Zongping, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009. Induction of cytoprotective autophagy in PC-12 cells by cadmium. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.07.050.
Wang, Qiwen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Bijie Pilot Area Research Institute of Bijie University, Bijie 551700, Zhu, Jiaqiao, Zhang, Kangbao, Jiang, Chenyang, Wang, Yi, Yuan, Yan, Bian, Jianchun, Liu, Xuezhong, Gu, Jianhong, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Liu, Zongping, & Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009. Induction of cytoprotective autophagy in PC-12 cells by cadmium. United States. https://doi.org/10.1016/J.BBRC.2013.07.050
Wang, Qiwen, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Bijie Pilot Area Research Institute of Bijie University, Bijie 551700, Zhu, Jiaqiao, Zhang, Kangbao, Jiang, Chenyang, Wang, Yi, Yuan, Yan, Bian, Jianchun, Liu, Xuezhong, Gu, Jianhong, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Liu, Zongping, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009. 2013. "Induction of cytoprotective autophagy in PC-12 cells by cadmium". United States. https://doi.org/10.1016/J.BBRC.2013.07.050.
@article{osti_22242055,
title = {Induction of cytoprotective autophagy in PC-12 cells by cadmium},
author = {Wang, Qiwen and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009 and Bijie Pilot Area Research Institute of Bijie University, Bijie 551700 and Zhu, Jiaqiao and Zhang, Kangbao and Jiang, Chenyang and Wang, Yi and Yuan, Yan and Bian, Jianchun and Liu, Xuezhong and Gu, Jianhong and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009 and Liu, Zongping and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009},
abstractNote = {Highlights: •Cadmium can promote early upregulation of autophagy in PC-12 cells. •Autophagy precedes apoptosis in cadmium-treated PC-12 cells. •Cadmium-induced autophagy is cytoprotective in PC-12 cells. •Class III PI3K/beclin-1/Bcl-2 signaling pathway plays a positive role in cadmium-triggered autophagy. -- Abstract: Laboratory data have demonstrated that cadmium (Cd) may induce neuronal apoptosis. However, little is known about the role of autophagy in neurons. In this study, cell viability decreased in a dose- and time-dependent manner after treatment with Cd in PC-12 cells. As cells were exposed to Cd, the levels of LC3-II proteins became elevated, specific punctate distribution of endogenous LC3-II increased, and numerous autophagosomes appeared, which suggest that Cd induced a high level of autophagy. In the late stages of autophagy, an increase in the apoptosis ratio was observed. Likewise, pre-treatment with chloroquine (an autophagic inhibitor) and rapamycin (an autophagic inducer) resulted in an increased and decreased percentage of apoptosis in contrast to other Cd-treated groups, respectively. The results indicate that autophagy delayed apoptosis in Cd-treated PC-12 cells. Furthermore, co-treatment of cells with chloroquine reduced autophagy and cell activity. However, rapamycin had an opposite effect on autophagy and cell activity. Moreover, class III PI3 K/beclin-1/Bcl-2 signaling pathways served a function in Cd-induced autophagy. The findings suggest that Cd can induce cytoprotective autophagy by activating class III PI3 K/beclin-1/Bcl-2 signaling pathways. In sum, this study strongly suggests that autophagy may serve a positive function in the reduction of Cd-induced cytotoxicity.},
doi = {10.1016/J.BBRC.2013.07.050},
url = {https://www.osti.gov/biblio/22242055}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 438,
place = {United States},
year = {Fri Aug 16 00:00:00 EDT 2013},
month = {Fri Aug 16 00:00:00 EDT 2013}
}