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Title: Characterization of the oncogenic function of centromere protein F in hepatocellular carcinoma

Highlights: •Overexpression of CENPF is frequently detected in HCC. •Upregulation of CENPF serves as an independent prognosis factor in HCC patients. •CENPF functions as an oncogene in HCC by promoting cell G2/M transition. -- Abstract: Centromere protein F (CENPF) is an essential nuclear protein associated with the centromere-kinetochore complex and plays a critical role in chromosome segregation during mitosis. Up-regulation of CENPF expression has previously been detected in several solid tumors. In this study, we aim to study the expression and functional role of CENPF in hepatocellular carcinoma (HCC). We found CENPF was frequently overexpressed in HCC as compared with non-tumor tissue. Up-regulated CENPF expression in HCC was positively correlated with serum AFP, venous invasion, advanced differentiation stage and a shorter overall survival. Cox regression analysis found that overexpression of CENPF was an independent prognosis factor in HCC. Functional studies found that silencing CENPF could decrease the ability of the cells to proliferate, form colonies and induce tumor formation in nude mice. Silencing CENPF also resulted in the cell cycle arrest at G2/M checkpoint by down-regulating cell cycle proteins cdc2 and cyclin B1. Our data suggest that CENPF is frequently overexpressed in HCC and plays a critical role in drivingmore » HCC tumorigenesis.« less
Authors:
; ; ;  [1] ;  [2] ;  [3] ; ;  [1] ;  [3] ;  [4] ;  [1] ;  [4] ;  [4]
  1. State Key Laboratory of Oncology in Southern China, Sun Yat-Sen University Cancer Center, Guangzhou (China)
  2. Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou (China)
  3. Department of Clinical Oncology, The University of Hong Kong, Pokfulam, Hong Kong (China)
  4. (China)
Publication Date:
OSTI Identifier:
22239667
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 436; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL CYCLE; CENTROMERES; CHROMOSOMES; DNA; EOSIN; HEMATOXYLIN; HEPATOMAS; MICE; MITOSIS; ONCOGENES; POLYMERASE CHAIN REACTION; PROTEINS; REGRESSION ANALYSIS; RNA; SERINE; THREONINE; TRANSLOCATION