skip to main content

Title: Development of an adaptable coherent x-ray diffraction microscope with the emphasis on imaging hydrated specimens

This paper describes the development of a versatile coherent x-ray diffraction microscope capable of imaging biological specimens in solution. The microscope is a flexible platform accommodating various conditions, from low vacuum (10{sup −2} Pa) to helium gas filled ambient pressure. This flexibility greatly expands the application area, from in situ materials science to biology systems in their native state, by significantly relaxing restrictions to the sample environment. The coherent diffraction microscope has been used successfully to image a yeast cell immersed in buffer solution. We believe that the design of this coherent diffraction microscope can be directly adapted to various platforms such as table top soft x-ray laser, synchrotron x-ray sources, and x-ray free electron laser with minor relevant adjustments.
Authors:
 [1] ;  [2] ; ; ; ; ; ; ;  [1] ;  [1] ;  [3]
  1. RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan)
  2. (Korea, Republic of)
  3. (United Kingdom)
Publication Date:
OSTI Identifier:
22224093
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 84; Journal Issue: 11; Other Information: (c) 2013 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; BIOLOGY; DESIGN; FREE ELECTRON LASERS; HELIUM; IMAGES; MATHEMATICAL SOLUTIONS; MICROSCOPES; SOFT X RADIATION; SOLUTIONS; SYNCHROTRONS; X-RAY DIFFRACTION; X-RAY SOURCES; YEASTS